Study of the biotransformation of a potential benzo[c]fluorene antineoplastic using high-performance liquid chromatography with high-speed-scanning ultraviolet detection
| Autor: | Milan Nobilis, Pavel Anzenbacher, František Trejtnar, Jaroslav Květina, Kamil Hrubý, Jiří Pastera, Karel Ubik, Zbyněk Svoboda |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Metabolite Antineoplastic Agents In Vitro Techniques Mass spectrometry High-performance liquid chromatography chemistry.chemical_compound Biotransformation medicine Animals Bile Rats Wistar Cells Cultured Chromatography High Pressure Liquid Fluorenes Chromatography General Chemistry Metabolism Benzo(c)fluorene Rats Perfusion Metabolic pathway medicine.anatomical_structure Liver chemistry Biochemistry Hepatocyte Carcinogens Spectrophotometry Ultraviolet |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 681:143-151 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(95)00523-4 |
| Popis: | As the sum of benfluron metabolites found was only a part of the total amount applied, a search for undiscovered metabolites was undertaken in the extracts from isolated rat hepatocytes and in the bile and perfusate in the experiments with an isolated perfused rat liver. To identify the metabolites, high-performance liquid chromatography with UV spectral analysis was used, as benfluron derivatives exhibit characteristic absorption spectra. Administration of known metabolites to experimental animals and selective induction of certain metabolic pathways led to the finding of new metabolites and of the respective conjugates. Fast atom bombardment-mass spectrometry analysis was used to identify the newly found metabolites and conjugates. |
| Databáze: | OpenAIRE |
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