Myristoylation profiling in human cells and zebrafish
| Autor: | Remigiusz A. Serwa, Malgorzata Broncel, Anthony I. Magee, Margaret J. Dallman, Eberhard Krause, Edward W. Tate, Paulina Ciepla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Multidisciplinary
biology Tagging by substrate Modification site Myristoylation Protein modification HEK 293 cells Myristic acid Protein lipidation biology.organism_classification lcsh:Computer applications to medicine. Medical informatics Combinatorial chemistry HeLa chemistry.chemical_compound chemistry Biochemistry Proteome lcsh:R858-859.7 Azide lcsh:Science (General) Zebrafish Data Article lcsh:Q1-390 |
| Zdroj: | Data in Brief, Vol 4, Iss C, Pp 379-383 (2015) Data in Brief, 4: 379-383 Data in Brief |
| ISSN: | 2352-3409 |
| Popis: | Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed. |
| Databáze: | OpenAIRE |
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