The suppression of tomato defence response genes upon potato cyst nematode infection indicates a key regulatory role of miRNAs
| Autor: | Piotr Cieszyński, Waldemar Skowron, Marek D. Koter, Marcin Filipecki, Mateusz Matuszkiewicz, Joanna Dąbrowska-Bronk, Magdalena Święcicka |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Globodera rostochiensis Arabidopsis Potato cyst nematode Context (language use) Plant Science Genes Plant 01 natural sciences Plant Roots Transcriptome 03 medical and health sciences Suppression Genetic Solanum lycopersicum Gene Expression Regulation Plant Botany Genetics Arabidopsis thaliana Animals Tylenchoidea Amplified Fragment Length Polymorphism Analysis Gene Disease Resistance Plant Diseases Solanum tuberosum biology Base Sequence Gene Expression Profiling fungi food and beverages biology.organism_classification Gene expression profiling MicroRNAs 030104 developmental biology Protein Kinases 010606 plant biology & botany |
| Zdroj: | Plant physiology and biochemistry : PPB. 113 |
| ISSN: | 1873-2690 |
| Popis: | Potato cyst nematode Globodera rostochiensis is an obligate parasite of solanaceous plants, triggering metabolic and morphological changes in roots which may result in substantial crop yield losses. Previously, we used the cDNA-AFLP to study the transcriptional dynamics in nematode infected tomato roots. Now, we present the rescreening of already published, upregulated transcript-derived fragment dataset using the most current tomato transcriptome sequences. Our reanalysis allowed to add 54 novel genes to 135, already found as upregulated in tomato roots upon G. rostochiensis infection (in total - 189). We also created completely new catalogue of downregulated sequences leading to the discovery of 76 novel genes. Functional classification of candidates showed that the 'wound, stress and defence response' category was enriched in the downregulated genes. We confirmed the transcriptional dynamics of six genes by qRT-PCR. To place our results in a broader context, we compared the tomato data with Arabidopsis thaliana, revealing similar proportions of upregulated and downregulated genes as well as similar enrichment of defence related transcripts in the downregulated group. Since transcript suppression is quite common in plant-nematode interactions, we assessed the possibility of miRNA-mediated inverse correlation on several tomato sequences belonging to NB-LRR and receptor-like kinase families. The qRT-PCR of miRNAs and putative target transcripts showed an opposite expression pattern in 9 cases. These results together with in silico analyses of potential miRNA targeting to the full repertoire of tomato R-genes show that miRNA mediated gene suppression may be a key regulatory mechanism during nematode parasitism. |
| Databáze: | OpenAIRE |
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