The morphological relationship in electron microscopy between NOR-silver proteins and intranucleolar chromatin
| Autor: | Massimo Derenzini, Daniele Hernandez-Verdun, M. Bouteille |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Nucleolus
Human cell line Biology Silver Proteins law.invention Cell Line chemistry.chemical_compound law Genetics Humans Genetics (clinical) Staining and Labeling Nuclear Proteins Chromatin Cell biology Staining Microscopy Electron Nucleoproteins chemistry Ribonucleoproteins Electron microscope Developmental biology Nucleophosmin DNA Cell Nucleolus |
| Zdroj: | Chromosoma. 85(4) |
| ISSN: | 0009-5915 |
| Popis: | The relative distribution of NOR proteins and chromatin fibers in the nucleoli was visualized in human cell line. The chromatin was revealed by a Feulgen-like procedure using osmium-ammine as DNA tracer. This selective staining was combined with NOR-silver staining. We provide morphological evidence for constant overlapping of the silver deposit sites with dispersed intranucleolar chromatin fibers. Silver stained proteins were sometimes observed in contact with the chromatin fibers, suggesting that at least some of the Ag-NOR proteins might be closely connected with the dispersed nucleolar DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink