Direct Detection ofMycobacterium bovisin Bovine Lymph Nodes by PCR
| Autor: | W. Okano, Maria Valdrinez Campana Lonardoni, Adolfo Carlos Barreto Santos, Clarice Queico Fujimura Leite, Noeme Sousa Rocha, Rosario Dominguez Crespo Hirata, Marco Antonio Cardoso, R. F. Cardoso, Vera Lúcia Dias Siqueira, M.H. Hirata |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Pathology medicine.medical_specialty Tuberculosis Epidemiology Tuberculin Biology Polymerase Chain Reaction law.invention law Zoonoses medicine Animals Humans Lymph node Polymerase chain reaction Bacteriological Techniques Mycobacterium bovis General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health medicine.disease biology.organism_classification DNA extraction Molecular biology Staining Infectious Diseases medicine.anatomical_structure Cattle Lymph Nodes Lymph Tuberculosis Bovine |
| Zdroj: | Zoonoses and Public Health. 56:465-470 |
| ISSN: | 1863-2378 1863-1959 |
| DOI: | 10.1111/j.1863-2378.2008.01199.x |
| Popis: | Thirty-five lymph node samples were taken from animals with macroscopic lesions consistent with Mycobacterium bovis infection. The animals were identified by postmortem examination in an abattoir in the northwestern region of state of Parana, Brazil. Twenty-two of the animals had previously been found to be tuberculin skin test positive. Tissue samples were decontaminated by Petroff's method and processed for acid-fast bacilli staining, culture in Stonebrink and Lowenstein-Jensen media and DNA extraction. Lymph node DNA samples were amplified by PCR in the absence and presence (inhibitor controls) of DNA extracted from M. bovis culture. Mycobacterium bovis was identified in 14 (42.4%) lymph node samples by both PCR and by culture. The frequency of PCR-positive results (54.5%) was similar to that of culture-positive results (51.5%, P > 0.05). The percentage of PCR-positive lymph nodes increased from 39.4% (13/33) to 54.5% (18/33) when samples that were initially PCR-negative were reanalysed using 2.5 microl DNA (two samples) and 1 : 2 diluted DNA (three samples). PCR sensitivity was affected by inhibitors and by the amount of DNA in the clinical samples. Our results indicate that direct detection of M. bovis in lymph nodes by PCR may be a fast and useful tool for bovine tuberculosis epidemic management in the region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|