Longitudinal relationships between rheumatoid factor and cytokine expression by immunostimulated peripheral blood lymphocytes from patients with rheumatoid arthritis: New insights into B-cell activation
| Autor: | Eric L. Matteson, Cynthia S. Crowson, Michael A. Strausbauch, John M. Davis, Sherine E. Gabriel, Peter J. Wettstein, Keith L. Knutson, Sara J. Achenbach, Terry M. Therneau |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chemokine Cell type medicine.drug_class medicine.medical_treatment Immunology Immunostimulant Article Arthritis Rheumatoid 03 medical and health sciences 0302 clinical medicine Rheumatoid Factor medicine Humans Immunology and Allergy Rheumatoid factor Lymphocytes Cells Cultured Aged B-Lymphocytes biology business.industry Middle Aged medicine.disease In vitro 030104 developmental biology Cytokine CpG site Rheumatoid arthritis biology.protein Cytokines business 030215 immunology |
| Zdroj: | Clin Immunol |
| ISSN: | 1521-6616 |
| Popis: | To identify associations between immunostimulated cytokine production and disease characteristics, peripheral blood lymphocytes were collected from 155 adult patients with rheumatoid arthritis (RA) before and after a 5-year interval. The lymphocytes were activated in vitro with T-cell stimulants, cytosine-phosphate-guanine (CpG) oligonucleotide, and medium alone (negative control). Expression of 17 cytokines was evaluated with immunoassays, and factor analysis was used to reduce data complexity and identify cytokine combinations indicative of cell types preferentially activated by each immunostimulant. The findings showed that the highest numbers of correlations were between cytokine levels and rheumatoid factor (RF) positivity and between cytokine levels and disease duration. Scores for cytokines driven by CpG and medium alone were negatively associated with RF positivity and disease duration at baseline but positively associated with both at 5 years. Our findings suggest that RF expression sustained over time increases activation of B cells and monocytes without requirements for T-cell functions. |
| Databáze: | OpenAIRE |
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