Preparation of β-XIIa (Hageman factor fragment) from human plasma
| Autor: | Barbara M. Alving, Donald L. Tankersley, J.S. Finlayson |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Factor XII
Chromatography Chemistry Goats Prekallikrein Hematology Kallikrein Blood Coagulation Disorders Factor XIIa Chromatography Ion Exchange Peptide Fragments Plasma Adsorption Human plasma Yield (chemistry) Chromatography Gel Animals Humans Electrophoresis Polyacrylamide Gel Rabbits Immunoelectrophoresis circulatory and respiratory physiology |
| Zdroj: | Thrombosis Research. 25:307-317 |
| ISSN: | 0049-3848 |
| Popis: | beta-Factor XIIa (beta-XIIa, Mr approximately 30,000) was isolated from human plasma by a procedure which utilized, as an initial step, the adsorption of Factor XII to celite. Activation of Factor XII and subsequent release of beta-XIIa was brought about by the proteolytic action of co-adsorbed kallikrein. Two successive chromatographic procedures were then used to achieve a final purification of 4,420-fold over plasma and an overall field of 2.3 mg of beta-XIIa per liter. |
| Databáze: | OpenAIRE |
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