Phosphoproteomic analysis reveals plant DNA damage signalling pathways with a functional role for histone H2AX phosphorylation in plant growth under genotoxic stress
| Autor: | Wanda M. Waterworth, Thomas S. Nühse, Christopher E. West, Dapeng Wang, Julian N. Selley, Stacey Warward, Michael Wilson |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Aging DNA Repair Proteome DNA repair DNA damage ATAXIA TELANGIECTASIA MUTATED (ATM) Mutant Arabidopsis Germination Plant Science Genotoxic Stress DNA damage response 01 natural sciences Mass Spectrometry Histones 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Serine Genetics Phosphorylation Cells Cultured biology Arabidopsis Proteins X-Rays Phosphoproteomics Histone H2AX food and beverages Original Articles Cell Biology Cell biology Gene Ontology 030104 developmental biology Histone Seeds biology.protein Original Article ATP-Binding Cassette Transporters seed DNA Damage Signal Transduction 010606 plant biology & botany |
| Zdroj: | Waterworth, W, Wilson, M, Wang, D, Nuhse, T, Warwood, S, Selley, J & West, C E 2019, ' Phosphoproteomic analysis reveals plant DNA damage signalling pathways with a functional role for Histone H2AX phosphorylation in plant growth under genotoxic stress ', The Plant journal : for cell and molecular biology, vol. 100, pp. 1007-1021 . https://doi.org/10.1111/tpj.14495 The Plant Journal |
| ISSN: | 0960-7412 |
| Popis: | Summary DNA damage responses are crucial for plant growth under genotoxic stress. Accumulating evidence indicates that DNA damage responses differ between plant cell types. Here, quantitative shotgun phosphoproteomics provided high‐throughput analysis of the DNA damage response network in callus cells. MS analysis revealed a wide network of highly dynamic changes in the phosphoprotein profile of genotoxin‐treated cells, largely mediated by the ATAXIA TELANGIECTASIA MUTATED (ATM) protein kinase, representing candidate factors that modulate plant growth, development and DNA repair. A C‐terminal dual serine target motif unique to H2AX in the plant lineage showed 171‐fold phosphorylation that was absent in atm mutant lines. The physiological significance of post‐translational DNA damage signalling to plant growth and survival was demonstrated using reverse genetics and complementation studies of h2ax mutants, establishing the functional role of ATM‐mediated histone modification in plant growth under genotoxic stress. Our findings demonstrate the complexity and functional significance of post‐translational DNA damage signalling responses in plants and establish the requirement of H2AX phosphorylation for plant survival under genotoxic stress. Significance Statement This work demonstrates the central role for ATM in DNA damage signalling and establishes the functional requirement for H2AX phosphorylation in plant resistance to genotoxic stress. These results revealed the physiological significance of ATM‐dependent post‐translational protein modification in the plant DNA damage response in mitigating the deleterious cellular consequences of genome damage. |
| Databáze: | OpenAIRE |
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