Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis
| Autor: | Daniela da Silva Nunes, Jair P. Cunha-Junior, Henrique Tomaz Gonzaga, Vanessa da Silva Ribeiro, Julia Maria Costa-Cruz |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Swine Immunoblotting Neurocysticercosis Antibodies Helminth Enzyme-Linked Immunosorbent Assay Chemical Fractionation Sensitivity and Specificity Serology Taenia solium medicine Animals Humans Serologic Tests Saline extract Chromatography General Veterinary biology Chemical fractionation General Medicine Chromatography Ion Exchange Metacestode medicine.drug_formulation_ingredient Infectious Diseases Ethanolamines Antigens Helminth Immunoglobulin G Insect Science biology.protein Parasitology Antibody |
| Zdroj: | Parasitology Research. 113:2569-2575 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5 % and DEAE S2 90 %. The best specificity was observed for DEAE S2 (90.4 %). In IB, using DEAE S2 samples from NC patients presented bands of 20–25, 43–45, 55–50, 60–66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis. |
| Databáze: | OpenAIRE |
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