Binary Colloidal Crystal Layers as Platforms for Surface Patterning of Puroindoline-Based Antimicrobial Peptides
| Autor: | Snehal R. Jadhav, Andrew Boden, Mrinal Bhave, Peng-Yuan Wang, Peter Kingshott |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Antimicrobial peptides Nanoparticle 02 engineering and technology Colloidal crystal 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Anti-Bacterial Agents chemistry.chemical_compound Surface coating Adsorption chemistry Chemical engineering Covalent bond Biofilms Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Escherichia coli Surface modification General Materials Science 0210 nano-technology Peptides Carbodiimide |
| Zdroj: | ACS applied materialsinterfaces. 10(3) |
| ISSN: | 1944-8252 |
| Popis: | The ability of bacteria to form biofilms and the emergence of antibiotic-resistant strains have prompted the need to develop the next generation of antibacterial coatings. Antimicrobial peptides (AMPs) are showing promise as molecules that can address these issues, especially if used when immobilized as a surface coating. We present a method that explores how surface patterns together with the selective immobilization of an AMP called PuroA (FPVTWRWWKWWKG-NH2) can be used to both kill bacteria and also as a tool to study bacterial attachment mechanisms. Surface patterning is achieved using stabilized self-assembled binary colloidal crystal (BCC) layers, allowing selective PuroA immobilization to carboxylated particles using N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide (EDC) hydrochloride/N-hydroxysuccinimide (NHS) coupling chemistry. Covalent immobilization of PuroA was compared with physical adsorption (i.e., without the addition of EDC/NHS). The AMP-functionalized colloids and BCC layers were charact... |
| Databáze: | OpenAIRE |
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