Mild Hypothermia Combined with Hydrogen Sulfide Treatment During Resuscitation Reduces Hippocampal Neuron Apoptosis Via NR2A, NR2B, and PI3K-Akt Signaling in a Rat Model of Cerebral Ischemia-Reperfusion Injury

Autor: Hai-bin Dai, Sihai Zhu, Ru-Meng Ma, Miao-miao Xu, Manlin Duan, Xiang-Jun Ji, Xiao-Lei Miao, Jia Lv
Rok vydání: 2015
Předmět:
Male
0301 basic medicine
Resuscitation
medicine.medical_treatment
Intraperitoneal injection
Neuroscience (miscellaneous)
Ischemia
Apoptosis
Sodium hydrosulfide
Pharmacology
Hippocampus
Receptors
N-Methyl-D-Aspartate
Brain Ischemia
Rats
Sprague-Dawley
Phosphatidylinositol 3-Kinases
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Hydroxylamine
Hypothermia
Induced
Animals
Medicine
Hydrogen Sulfide
Saline
Neurons
business.industry
Hypothermia
medicine.disease
Combined Modality Therapy
Rats
Disease Models
Animal
Treatment Outcome
030104 developmental biology
Neurology
chemistry
Reperfusion Injury
Anesthesia
medicine.symptom
business
Proto-Oncogene Proteins c-akt
Reperfusion injury
030217 neurology & neurosurgery
Signal Transduction
Zdroj: Molecular Neurobiology. 53:4865-4873
ISSN: 1559-1182
0893-7648
DOI: 10.1007/s12035-015-9391-z
Popis: We investigated whether mild hypothermia combined with sodium hydrosulfide treatment during resuscitation improves neuron survival following cerebral ischemia-reperfusion injury beyond that observed for the individual treatments. Male Sprague-Dawley rats were divided into seven groups (n = 20 for each group). All rats underwent Pulsinelli 4-vessel occlusion. Ischemia was induced for 15 min using ligatures around the common carotid arteries, except for the sham group. Immediately after initiating reperfusion, the mild hypothermia (MH), sodium hydrosulfide (NaHS), hydroxylamine (HA), MH + NaHS, MH + HA, and ischemia-reperfusion (I/R) control groups received an intraperitoneal injection of saline, sodium hydrosulfide, hydroxylamine, sodium hydrosulfide, hydroxylamine, and saline, respectively, and mild hypothermia (32 to 33 °C) was induced in the MH, MH + NaHS, and MH + HA groups for 6 h. The levels of NR2A, NR2B, p-Akt, and p-Gsk-3β in the hippocampus of the MH, NaHS, and MH + NaHS groups were higher than those in the I/R control group, with the highest levels observed in the MH + NaHS group (P
Databáze: OpenAIRE
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