Immunocompetence and alterations in hepatic gene expression in rainbow trout exposed to CdS/CdTe quantum dots
| Autor: | G. van Aggelen, L. Yu, François Gagné, Michel Fournier, Heather L. Osachoff, Christian Gagnon, M. Fortier, Rachel C. Skirrow |
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| Přispěvatelé: | Fluvial Ecosystem Research, Aquatic Ecosystem Research Protection, Water Science and Technology, Environment and Climate Change Canada, Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Pacific and Yukon Laboratory for Environmental Testing, Water Science and Technology |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Gene Expression
010501 environmental sciences Kidney 01 natural sciences MESH: Dose-Response Relationship Drug chemistry.chemical_compound Leukocyte Count Gene expression Metallothionein Inducer MESH: Animals MESH: Phagocytosis chemistry.chemical_classification 0303 health sciences Cadmium biology General Medicine MESH: Quantum Dots MESH: Water Pollutants Chemical Biochemistry Liver MESH: Tellurium MESH: Oncorhynchus mykiss Oncorhynchus mykiss [SDV.TOX]Life Sciences [q-bio]/Toxicology Immunocompetence Tellurium Fish Proteins MESH: Gene Expression MESH: Cadmium chemistry.chemical_element Management Monitoring Policy and Law 03 medical and health sciences Vitellogenin Phagocytosis Quantum Dots MESH: Fish Proteins Animals 14. Life underwater 030304 developmental biology 0105 earth and related environmental sciences Reactive oxygen species Dose-Response Relationship Drug Public Health Environmental and Occupational Health MESH: Kidney MESH: Immunocompetence chemistry MESH: Leukocyte Count Immunology biology.protein Xenobiotic Water Pollutants Chemical MESH: Liver |
| Zdroj: | Journal of Environmental Monitoring Journal of Environmental Monitoring, Royal Society of Chemistry, 2010, 12 (8), pp.1556-65. ⟨10.1039/c0em00031k⟩ |
| ISSN: | 1464-0325 1464-0333 |
| DOI: | 10.1039/c0em00031k⟩ |
| Popis: | International audience; Cadmium (Cd) tellurium quantum dots (QDs) are long-lived fluorescent nanocrystals that have the ability to produce potentially toxic reactive oxygen species at the surface of the nanoparticle and release toxic cadmium ions. The purpose of this study was to examine the sublethal effects of CdS/CdTe QDs and dissolved Cd on the immune system of rainbow trout. Changes in hepatic gene expression were also monitored to provide insight on the mode of action of both forms of cadmium. Oncorhynchus mykiss fish were exposed to increasing concentrations of CdS/CdTe or dissolved Cd (CdSO(4)) for 48 h at 15 degrees C. The anterior head kidney was analyzed for leukocyte count, viability and phagocytic activity. The livers were harvested and prepared for gene expression analysis using a DNA microarray comprised of 207 stress-related genes. An analysis of total Cd in the aquarium water revealed that the nominal concentrations corresponded well to the actual concentrations and that a small proportion (0.4%) of Cd in the QDs was found in the dissolved fraction. Exposure to QDs led to significant reductions in leukocyte counts, viability and both resting and active phagocytic activity. On a mass concentration basis, QDs were more potent than dissolved Cd in suppressing immunocompetence in rainbow trout. The analysis revealed that both forms of Cd were strong inducers of metallothionein and CP2K1 gene expressions, which are respectively involved in metal detoxification processes and xenobiotic transformation/inflammation conditions. The analysis revealed different modes of action for each form of Cd. For QDs, 25 genes specific to QDs and related to the immune endpoints were found. The genes were involved in inflammation, xenobiotic biotransformation and endocrine system (including the induction of vitellogenin and its receptor). The effect for dissolved Cd was narrower than for QDs, with 9 genes specific to dissolved Cd being strongly correlated with the observed effects on immunocompetence. The involved genes were binding and transport of various solutes (urea, sodium, potassium) and the complement system. The present study revealed that each form of Cd produced a different pattern of gene expression and lowered fish immunocompetence. |
| Databáze: | OpenAIRE |
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