Use of Cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by Clostridium beijerinckii
| Autor: | Venkat Gopalan, Thaddeus Chukwuemeka Ezeji, Victor Ujor, Chidozie Victor Agu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Butanols Bioengineering Poaceae Furfural Lignin Applied Microbiology and Biotechnology Syringaldehyde Acetone 03 medical and health sciences chemistry.chemical_compound Vanillic acid Biomass Food science Clostridium beijerinckii Ethanol biology Chemistry Cupriavidus basilensis Butanol Cupriavidus biology.organism_classification 030104 developmental biology Biochemistry Fermentation Biotechnology |
| Zdroj: | Journal of Industrial Microbiology and Biotechnology. 43:1215-1226 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-016-1798-7 |
| Popis: | Lignocellulose-derived microbial inhibitors (LDMICs) prevent efficient fermentation of Miscanthus giganteus (MG) hydrolysates to fuels and chemicals. To address this problem, we explored detoxification of pretreated MG biomass by Cupriavidus basilensis ATCC®BAA-699 prior to enzymatic saccharification. We document three key findings from our test of this strategy to alleviate LDMIC-mediated toxicity on Clostridium beijerinckii NCIMB 8052 during fermentation of MG hydrolysates. First, we demonstrate that growth of C. basilensis is possible on furfural, 5-hydroxymethyfurfural, cinnamaldehyde, 4-hydroxybenzaldehyde, syringaldehyde, vanillin, and ferulic, p-coumaric, syringic and vanillic acid, as sole carbon sources. Second, we report that C. basilensis detoxified and metabolized ~98 % LDMICs present in dilute acid-pretreated MG hydrolysates. Last, this bioabatement resulted in significant payoffs during acetone-butanol-ethanol (ABE) fermentation by C. beijerinckii: 70, 50 and 73 % improvement in ABE concentration, yield and productivity, respectively. Together, our results show that biological detoxification of acid-pretreated MG hydrolysates prior to fermentation is feasible and beneficial. |
| Databáze: | OpenAIRE |
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