Comparison between virulence characteristics of dominant and non-dominant Escherichia coli strains of the gut and their interaction with Caco-2 cells
| Autor: | N Masters, Christian O'Dea, Mohammad Katouli, Anna Kuballa, Tara L. Vollmerhausen, Bahareh Owrangi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine 030106 microbiology Population Porins Virulence Biology medicine.disease_cause Microbiology Bacterial Adhesion Feces 03 medical and health sciences Sex Factors Escherichia coli Prevalence medicine Humans Allele education Escherichia coli Infections Adhesins Escherichia coli education.field_of_study Escherichia coli Proteins Biofilm Epithelial Cells Gut Epithelium Gastrointestinal Tract Intestinal Diseases 030104 developmental biology Infectious Diseases Caco-2 Bacterial Translocation Biofilms Host-Pathogen Interactions ATP-Binding Cassette Transporters Female Fimbriae Proteins Caco-2 Cells |
| Zdroj: | Microbial Pathogenesis. 105:171-176 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2017.02.032 |
| Popis: | Escherichia coli strains are normal inhabitants of the gut and are normally found in the faeces of the host at different population sizes. We characterised faecal E. coli of 45 healthy male (n = 17) and female (n = 28) volunteers by testing 28 isolates from each individual. These isolates were typed and divided into dominant (if constituted >50% of the population tested) and non-dominant types in each individual. Representative strains of each dominant and non-dominant type were tested for their virulence gene profiles, their ability to form biofilm, adhere to, invade and translocate through a gut epithelial cell line (Caco-2 cells). Strains belonging to dominant types adhered significantly more to Caco-2 cells than non-dominant strains (5.7 ± 0.3 versus 4.3.± 0.13 CFU/cell mean ± SEM, P = 0.0003). They also invaded (135 ± 6 versus 63 ± 13 CFU) and translocated through Caco-2 cells (84 ± 5 versus 32 ± 9 CFU) significantly more than non-dominant strains (P < 0.0001 and P = 0.0002, respectively). Moreover, dominant strains showed the ability to form significantly more biofilm than non-dominant strains (1.1 ± 0.01 versus 0.5 ± 0.1 OD600, P < 0.0001). Majority (51%) of the strains belonged to phylogroup D followed by B2 (23%). Furthermore, out of 25 virulence genes tested, kpsMTII, papC and papG allele III were found to be significantly higher among dominant than non-dominant strains. Our results suggest that E. coli strains dominating the gut may have virulence properties that enable them to efficiently interact with the gut epithelium and translocate under predisposing conditions of the host. |
| Databáze: | OpenAIRE |
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