Effect of ovarian aging on androgen biosynthesis in a cynomolgus macaque model
| Autor: | Kelly F. Ethun, Jay R. Kaplan, S.E. Appt, Charles E. Wood, Haiying Chen, C. R. Parker |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
3-Hydroxysteroid Dehydrogenases medicine.drug_class Ovariectomy Ovary Macaque Article Follicle biology.animal Internal medicine Preoperative Care medicine Animals Ovarian reserve Coloring Agents Cellular Senescence Monitoring Physiologic Postoperative Care biology Theca interna Obstetrics and Gynecology Steroid 17-alpha-Hydroxylase General Medicine Androgen Immunohistochemistry Macaca fascicularis Endocrinology medicine.anatomical_structure Cytochromes b5 Theca Cells Models Animal Androgens Female Folliculogenesis Stromal Cells Cell aging |
| Zdroj: | Climacteric : the journal of the International Menopause Society. 15(1) |
| ISSN: | 1473-0804 |
| Popis: | The role of androgens in chronic disease pathogenesis, cognitive function and libido during menopause is of increasing interest. The aim of this study was to characterize the distribution and expression of androgenic proteins in the macaque ovary and to investigate the relationship between serum androgen concentrations, follicle number, and the persistence of androgenesis in the aging macaque ovary.The subjects were 26 adult female cynomolgus macaques. Ovaries were immunostained for cytochrome P450 17α-hydroxylase/17-20 lyase (P450c17), 3β-hydroxysteroid dehydrogenase (3βHSD), and cytochrome b5 (cytb5). Based on primordial follicle counts, animals were divided into tertiles (low (≤200), intermediate (226-1232), and high (2372-4356)) to evaluate differences in androgen staining and changes in serum androgen concentrations following ovariectomy.Positive immunostaining for P450c17 and cytb5 within the theca interna layer of growing follicles persisted in advanced atretic follicles and secondary interstitial cells (residual stromal cells). Ovaries with low follicle numbers had less staining for all androgenic proteins compared to ovaries with higher numbers of growing follicles. Immunostaining for cytb5 was the most reliable marker for persistent androgenesis in ovaries with minimal primordial follicle numbers (100) and residual stromal cells. Following ovariectomy, a significant decrease in testosterone (-27.7%, -30.8%, -27.5%; p0.01) and androstenedione (-33.4%, -35.7%, -46.0%; p0.01) was observed in monkeys with low, intermediate, and high primordial follicle counts, respectively.Despite low follicle numbers, the aging macaque ovary retains the necessary proteins for androgenesis within residual stromal cells and contributes to peripheral androgen concentrations. |
| Databáze: | OpenAIRE |
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