Low dose gamma irradiation pretreatment modulates the sensitivity of CNS to subsequent mixed gamma and neutron irradiation of the mouse head
| Autor: | Yulia Semochkina, O V Vysotskaya, A. V. Rodina, Aleksandr N. Strepetov, Elizaveta Yu. Moskaleva, Anastasia N. Romantsova |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Central Nervous System
medicine.medical_specialty Doublecortin Protein Neurogenesis Morris water navigation task Inflammation Hippocampal formation Open field 030218 nuclear medicine & medical imaging 03 medical and health sciences Mice 0302 clinical medicine Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Irradiation Neutron irradiation Neuroinflammation Neutrons Radiological and Ultrasound Technology Microglia Chemistry Dose-Response Relationship Radiation Endocrinology medicine.anatomical_structure Gamma Rays 030220 oncology & carcinogenesis Cytokines medicine.symptom Head |
| Zdroj: | International journal of radiation biology. 97(7) |
| ISSN: | 1362-3095 |
| Popis: | ABSTRACТPurpose: To explore if the total body γ-irradiation at a dose of 0.1 Gy 7 days prior to acute mixed γ, n-irradiation of the head at the dose of 1 Gy can reduce the harmful effects of neutron irradiation on the hippocampal functions, neuroinflammation and neurogenesis.Materials and methods: Mice were exposed to γ-radiation alone, mixed γ,n-radiation or combined γ-rays and γ,n-radiation 7 days after γ-irradiation. Two months post-irradiation, mice were tested in Open Field and in the Morris water maze. The content of microglia, astrocytes, proliferating cells and cytokines TGF-β, TNF-α, IL-1β, GFAP levels, hippocampal BDNF, NT-3, NT-4, NGF mRNA expression were evaluated.Results: Two months after combined irradiation, we observed impaired hippocampus-dependent cognition, which was not detected in mice exposed to γ,n-irradiation. Combined exposure and γ,n-irradiation led to a significant increase in the level of activated microglia and astrocytes in the brains. The level of pro- and anti-inflammatory cytokines in the brain and hippocampal neurotrophine's genes changed differenly after the combined exposure and γ,n-irradiation. The quantity of DCX-positive cells was reduced after γ,n-irradiation exposer alone, but increased after combined irradiation.Conclusions: Our results indicate radio-adaptive responses in brains of mice that were exposed to low-dose gamma irradiation 7 days prior to acute 1 Gy γ,n-irradiation. |
| Databáze: | OpenAIRE |
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