Diagnostic performance of routine electrophoresis and immunofixation for the detection of immunoglobulin paraproteins (M-Proteins) in dogs with multiple myeloma and related disorders: Part 2-Toward improved diagnostic performance
| Autor: | Christina Jeffries, Laura V. Ashton, A Russell Moore, R Adam Harris, Paul R. Avery |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Immunofixation
030213 general clinical medicine Pathology medicine.medical_specialty Globulin 040301 veterinary sciences Immunoglobulin light chain 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Capillary electrophoresis Dogs medicine Animals Dog Diseases Immunoelectrophoresis Multiple myeloma General Veterinary biology business.industry 04 agricultural and veterinary sciences medicine.disease Blood Protein Electrophoresis Agarose gel electrophoresis biology.protein Immunoglobulin Light Chains Paraproteins Antibody business Multiple Myeloma |
| Zdroj: | Veterinary clinical pathologyREFERENCES. 50(2) |
| ISSN: | 1939-165X |
| Popis: | Background The diagnostic performance of routine electrophoresis (agarose gel electrophoresis [AGE] and capillary zone electrophoresis [CZE]) and species-specific immunofixation (IF) for the detection of immunoglobulin paraproteins (M-proteins) and diagnosis of secretory myeloma-related disorders (sMRD) can be improved. Available canine IF targets were IgG-FC, IgA, IgM, light chain (LC), IgG4, and free LC (fLC) antibodies. Objective We aimed to review specific features associated with the presence of M-proteins in canine serum samples and the common features causing inaccurate reporting of M-proteins to improve the diagnostic performance of routine electrophoresis and IF for the detection of M-proteins. Methods Features found in AGE, CZE, routine IF, IgG4 IF, and fLC IF of 100 canine serum samples from Part 1 of this study were evaluated by simple and multivariate logistic regression to identify factors associated with the presence of M-proteins. Cases falsely called negative or positive for M-proteins were reviewed to identify the common features that could be used to increase the diagnostic performance of SPE and IF for M-protein detection. Results The presence of hypogammaglobulinemia or any peak taller than albumin was associated with an M-protein. Total protein concentrations, globulin concentrations, or peaks wider than albumin were not associated with an M-protein. Free LC sMRD cases were not diagnosed by SPE and routine IF. Cases with infectious and inflammatory etiologies had a restricted polyclonal gammopathy with multiple γ-globulin restrictions resulting in some false-positive results. SPE combined with all available IF results and the specific features identified in this study had an estimated sensitivity of 95.1% and specificity of 81.4%. Conclusions The identified criteria of this study increase the diagnostic performance of the electrophoretic evaluation for M-proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text