Comparing the role of silica particle size with mineral fiber geometry in the release of superoxide from rat alveolar macrophages
| Autor: | Takayuki Kameda, Masayuki Ohyama, Hideki Tachi, Chika Minejima |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Luminescence Kinetics Mineralogy Toxicology law.invention chemistry.chemical_compound Superoxides law Macrophages Alveolar Animals Fiber Particle Size Quartz Cells Cultured Chemiluminescence Mineral Fibers chemistry.chemical_classification Reactive oxygen species Mineral Superoxide respiratory system Particulates Oxidants Silicon Dioxide Rats Inbred F344 chemistry Chemical engineering Acridines Particulate Matter |
| Zdroj: | The Journal of Toxicological Sciences. 39:551-559 |
| ISSN: | 1880-3989 0388-1350 |
| DOI: | 10.2131/jts.39.551 |
| Popis: | Particulate air pollutants and mineral fibers activate inflammatory cells to release oxidants, which contribute to inflammation and injury in the lower respiratory tract. Our aim was to compare the role of silica particle size with mineral fiber length and width in the ability to induce superoxide release from rat alveolar macrophages. We estimated the ability of four types of silica particle samples, with different mode diameter, and three types of mineral fiber samples, with different geometric mean lengths and widths, to induce lucigenin-dependent chemiluminescence (CL) from the cells per number of dust particles (i.e., silica particles and mineral fibers). A close positive correlation was observed between dust size and the ability to induce CL in silica as well as mineral fiber samples. Moreover, the ability of silica samples to induce CL was weaker than that of long mineral fiber sample. This ability increased at a larger rate in small silica particle and thin mineral fiber samples than in large silica particle and thick mineral fiber samples at the initial stage of administration. These results suggest that the kinetics of the induction superoxide release from macrophages is similar between silica particles and mineral fibers; moreover, this depends on silica particle size and mineral fiber geometry. Finally, large silica particles were more active than small ones. |
| Databáze: | OpenAIRE |
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