Availability of culture filtrate protein-10 (CFP-10) secreted from Mycobacterium pseudoshottsii for mycobacteriosis diagnosis in ginbuna crucian carp Carrasius auratus langsdorfii
| Autor: | Kyosuke Araki, Atsushi Yamamoto, Kazuma Hayashi, Teruyuki Nakanishi, Megumi Matsumoto |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Veterinary (miscellaneous) Aquatic Science complex mixtures Microbiology Mycobacterium Mycobacterium tuberculosis 03 medical and health sciences Fish Diseases Immune system Plasmid Antigen Bacterial Proteins Goldfish medicine Animals Interferon gamma Amino Acid Sequence Phylogeny Antigens Bacterial Mycobacterium Infections CFP-10 biology 04 agricultural and veterinary sciences bacterial infections and mycoses biology.organism_classification 030104 developmental biology 040102 fisheries Crucian carp 0401 agriculture forestry and fisheries Mycobacterium pseudoshottsii Sequence Alignment medicine.drug |
| Zdroj: | Journal of fish diseasesREFERENCES. 43(1) |
| ISSN: | 1365-2761 |
| Popis: | Mycobacteriosis in cultured fish is a challenge for the aquaculture industry worldwide. Treatment by chemical administration is difficult and no effective vaccine has been developed. Therefore, detection and isolation by early diagnosis are important for prevention of the spread of the disease. In mammals, interferon gamma release assays have been established for detection of tuberculosis; these tests are based on the delayed-type hypersensitivity (DTH) response against culture filtrate protein-10 (CFP-10) and the 6-kDa early secreted antigen target (ESAT-6) of Mycobacterium tuberculosis. On the other hand, little is known about the fish immune response against the ESAT-6 and CFP-10 proteins of mycobacteria, although these responses should find application in the diagnosis of mycobacteriosis in fish. In the present study, we identified ESAT-6 and CFP-10 from Mycobacterium pseudoshottsii and cloned the corresponding genes. Intraperitoneal injection of the corresponding DNA plasmid constructs in ginbuna crucian carp yielded increased expression of the fish interferon-γ1-1-encoding gene (IFN-γ1-1). In contrast, IFN-γ1-1 expression accompanied by DTH response was observed only in the CFP-10-DNA plasmid-injected fish. Furthermore, fish that had been prophylactically injected with CFP-10-DNA plasmid exhibited increased survival of M. pseudoshottsii infection. Taken together, these results suggested that CFP-10 may facilitate diagnosis of mycobacteriosis. |
| Databáze: | OpenAIRE |
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