Effect of saponins from Helicteres isora on lipid and glucose metabolism regulating genes expression
| Autor: | Suresh Giri, Dev D. Santani, Shefalee K. Bhavsar, Satinder Singh, Mukul R. Jain |
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| Rok vydání: | 2009 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Mice Obese Peroxisome proliferator-activated receptor Plant Roots Mice Internal medicine Drug Discovery Diabetes Mellitus medicine Angiopoietin-Like Protein 4 Animals Insulin PPAR alpha RNA Messenger Malvaceae Angiopoietin-Like Protein 3 Pharmacology chemistry.chemical_classification Glucose tolerance test biology medicine.diagnostic_test Adiponectin Plant Extracts Methanol Glucose transporter Glucose Tolerance Test Saponins Lipid Metabolism Mice Inbred C57BL Angiopoietin-like Proteins Glucose Endocrinology Gene Expression Regulation Liver chemistry Solvents biology.protein Carbohydrate Metabolism GLUT2 Peroxisome proliferator-activated receptor alpha Angiopoietins Glucose 6-phosphatase GLUT4 |
| Zdroj: | Journal of Ethnopharmacology. 124:426-433 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2009.05.041 |
| Popis: | Ethnopharmacological relevance We characterized saponins as active constituents from traditionally used antidiabetic plant Helicteres isora . Aim of the study To evaluate the changes in the gene expression of the glucose and lipid metabolism regulating genes in C57BL/KsJ- db / db mice. Materials and methods C57BL/KsJ- db / db mice were divided into four different groups; one diabetic control, the mice in other three groups were treated with methanol extract (100 mg/kg), saponins (100 mg/kg) and pioglitazone (30 mg/kg) for 14 days. After completion of the treatment period biochemical parameters and the expression levels of adipsin, adiponectin, glucose transporter 4 (Glut4), peroxisome proliferator activated receptor gamma (PPARγ), fatty acid binding protein 4 (FABP4), lipoprotein lipase (LPL) in adipose tissue and for liver RNA samples glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), glucose transporter 2 (Glut2) and acyl-co-enzyme A oxidase (ACOX) were determined by quantitative real time PCR and angiopoeitin like 3 (ANGPTL3), angiopoeitin like 4 (ANGPTL4) and peroxisome proliferator activated receptor alpha (PPARα) by semiquantitative reverse transcription PCR. Results Treatment caused a significant reduction in the serum lipid and glucose levels and increased the expression of adipsin, PPARγ and Glut4 while reduced expression of FABP4 and G6Pase, whereas there was no effect on the expression levels of adiponectin, LPL, PEPCK, ACOX, Glut2, ANGPTL3, ANGPTL4 and PPARα. Conclusions Saponins are beneficial for improving hyperlipidemia and hyperglycemia by increasing the gene expression of adipsin, Glut4 and PPARγ and reducing the gene expression of the enzyme G6Pase and FABP4 in C57BL/KsJ- db / db mice. |
| Databáze: | OpenAIRE |
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