Ubiquitin Enzymes, Ubiquitin and Proteasome Activity in Blood Mononuclear Cells of MCI, Alzheimer and Parkinson Patients
| Autor: | Reinhilde Mlekusch, Josef Marksteiner, Christian Humpel, Alfred Kuschnig, Celine Ullrich |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Proteasome Endopeptidase Complex medicine.medical_specialty Ubiquitin-Activating Enzymes Disease Neuropsychological Tests Biology Peripheral blood mononuclear cell Statistics Nonparametric Western blot Ubiquitin Alzheimer Disease Internal medicine medicine Animals Chymotrypsin Humans Cognitive impairment Aged chemistry.chemical_classification Blood Cells medicine.diagnostic_test Neurodegeneration Parkinson Disease Middle Aged medicine.disease Rats Endocrinology Enzyme Proteasome activity chemistry Neurology Immunology biology.protein Female Neurology (clinical) Cognition Disorders Mental Status Schedule |
| Zdroj: | Current Alzheimer Research. 999:1-7 |
| ISSN: | 1567-2050 |
| DOI: | 10.2174/1567210204558642050 |
| Popis: | Alzheimer's disease (AD) is a severe chronic neurodegenerative disease. During aging and neurodegeneration, misfolded proteins accumulate and activate the ubiquitin-proteasome system. The aim of the present study is to explore whether ubiquitin-activating enzyme E1, ubiquitin-conjugating enzyme E2, ubiquitin or proteasome activity are affected in peripheral blood mononuclear cells (PBMC) of AD, mild cognitive impairment (MCI) and Parkinson's disease (PD) patients compared to healthy subjects. PBMCs were isolated from EDTA blood samples and extracts were analyzed by Western Blot. Proteasome activity was measured with fluorogenic substrates. When compared to healthy subjects, the concentration of enzyme E1 was increased in PBMCs of AD patients, whereas the concentration of the enzyme E2 was decreased in these same patients. Ubiquitin levels and proteasome activity were unchanged in AD patients. No changes in enzyme expression or proteasome activity was observed in MCI patients compared to healthy and AD subjects. In PD patients E2 levels and proteasomal activity were significantly reduced, while ubiquitin and E1 levels were unchanged. The present investigation demonstrates the differences in enzyme and proteasome activity patterns of AD and PD patients. These results suggest that different mechanisms are involved in regulating the ubiquitin-proteasomal system in different neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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