Glycidamide Promotes the Growth and Migratory Ability of Prostate Cancer Cells by Changing the Protein Expression of Cell Cycle Regulators and Epithelial-to-Mesenchymal Transition (EMT)-Associated Proteins with Prognostic Relevance

Autor: Chi Chen Huang, Wen Fu T. Lai, Titus Ime Ekanem, Kuen Haur Lee, Ding Yen Lin, Ming Heng Wu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Epithelial-Mesenchymal Transition
Cell Survival
Regulator
Cell Cycle Proteins
Kaplan-Meier Estimate
Biology
gene signature
Catalysis
Article
Malignant transformation
lcsh:Chemistry
Inorganic Chemistry
Prostate cancer
Cell Movement
Cell Line
Tumor
medicine
Biomarkers
Tumor
Humans
Epithelial–mesenchymal transition
cell cycle regulator
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Gene Expression Profiling
Organic Chemistry
Cell Cycle
fungi
EMT
Prostatic Neoplasms
food and beverages
General Medicine
Cell cycle
Gene signature
medicine.disease
prostate cancer
Computer Science Applications
Gene Expression Regulation
Neoplastic
SNAI2
lcsh:Biology (General)
lcsh:QD1-999
glycidamide
Cancer cell
Cancer research
Epoxy Compounds
prognosis
Transcriptome
Signal Transduction
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 9
International Journal of Molecular Sciences, Vol 20, Iss 9, p 2199 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20092199
Popis: Acrylamide (AA) and glycidamide (GA) can be produced in carbohydrate-rich food when heated at a high temperature, which can induce a malignant transformation. It has been demonstrated that GA is more mutagenic than AA. It has been shown that the proliferation rate of some cancer cells are increased by treatment with GA
however, the exact genes that are induced by GA in most cancer cells are not clear. In the present study, we demonstrated that GA promotes the growth of prostate cancer cells through induced protein expression of the cell cycle regulator. In addition, we also found that GA promoted the migratory ability of prostate cancer cells through induced epithelial-to-mesenchymal transition (EMT)-associated protein expression. In order to understand the potential prognostic relevance of GA-mediated regulators of the cell cycle and EMT, we present a three-gene signature to evaluate the prognosis of prostate cancer patients. Further investigations suggested that the three-gene signature (CDK4, TWIST1 and SNAI2) predicted the chances of survival better than any of the three genes alone for the first time. In conclusion, we suggested that the three-gene signature model can act as marker of GA exposure. Hence, this multi-gene panel may serve as a promising outcome predictor and potential therapeutic target in prostate cancer patients.
Databáze: OpenAIRE
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