Unexpected high testis-specific transcriptional activity of the cyclin T1 promoter in transgenic mice

Autor: Olivier Andreoletti, K Le Roux, J. Costa Da Silva, Xavier Mata, Gaëlle Tilly, M. Hudrisier, Nathalie Besnard, Jean-Luc Vilotte, Hubert Laude
Přispěvatelé: Mata, Xavier, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche de physiopathologie infectieuse et parasitaire des ruminants, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de recherche Virologie et Immunologie Moléculaires (VIM), Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), Unité associée de Physiopathologie respiratoire des ruminants
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Male
Transcription
Genetic
5' Flanking Region
Cyclin D
[SDV]Life Sciences [q-bio]
PRION
Endogeny
MESH: Microinjections
Biochemistry
Mice
Structural Biology
Transcription (biology)
MESH: Genetic Vectors
Testis
Cyclin T1 promoter
MESH: Animals
Tissue Distribution
Promoter Regions
Genetic
0303 health sciences
biology
MESH: Testis
Cyclin T
Goats
030302 biochemistry & molecular biology
3. Good health
MESH: Cattle
[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Transcription
Genetically modified mouse
Cyclin T1
Microinjections
MESH: Mice
Transgenic
Prions
Genetic Vectors
Biophysics
Mice
Transgenic
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Goats
PRNP
03 medical and health sciences
Cyclin D1
MESH: Prions
Transgenic mouse
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Cyclins
MESH: Promoter Regions
Genetic
Genetics
Animals
MESH: Tissue Distribution
Molecular Biology
Gene
MESH: Mice
030304 developmental biology
MESH: 5' Flanking Region
MESH: Transcription
Genetic
CYCLINE T1
Cell Biology
TRANSGENESIS
MESH: Cyclins
Molecular biology
MESH: Male
MESH: Germ Cells
Germ Cells
biology.protein
Cattle
MESH: Cyclin T
Zdroj: FEBS Letters
FEBS Letters, Wiley, 2003, 549 (1-3), pp.163-6
FEBS Letters, Wiley, 2003, 549, pp.163-166
FEBS Letters, Wiley, 2003, 549 (1-3), pp.163-166. ⟨10.1016/S0014-5793(03)00806-8⟩
ISSN: 0014-5793
1873-3468
DOI: 10.1016/S0014-5793(03)00806-8⟩
Popis: International audience; The ubiquitously expressed cyclin T1 gene encodes for a protein involved in human immunodeficiency virus type 1 (HIV-1) transcription activation. The goat gene was recently shown to share an expression pattern similar to that of its endogenous counterpart when incorporated into mice using a BAC insert. To assess if its promoter could target ubiquitous expression of the bovine Prnp in transgenic mice, two constructs carrying either 1 or 30 kb of cyclin T1 5'-flanking sequences were built and microinjected. Both constructs resulted in the unexpected high male germ cell-specific expression of the prion protein. These data re-question the suspected location of the cyclin T1 gene regulatory elements.
Databáze: OpenAIRE
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