Comparative analysis of minimal residual disease detection by multiparameter flow cytometry and enhanced ASO RQ-PCR in multiple myeloma
| Autor: | T-L Penttila, Tuomas Selander, Tuija Lundán, V. Huotari, Pekka Anttila, Veli Kairisto, Kari Remes, Mervi Putkonen, Lasse Uotila, T-T Pelliniemi, Vesa Juvonen, Pentti Mäntymaa, Sanna Siitonen, Raija Silvennoinen |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Neoplasm Residual Real-Time Polymerase Chain Reaction ta3111 Flow cytometry hemic and lymphatic diseases medicine TaqMan Humans Multiparameter flow cytometry Multiple myeloma Aged ASO-RQ-PCR medicine.diagnostic_test Oligonucleotide business.industry Hematology Middle Aged ta3121 Flow Cytometry medicine.disease ta3122 Minimal residual disease 3. Good health Real-time polymerase chain reaction Oncology Female Original Article Multiple Myeloma Nuclear medicine business |
| Zdroj: | Blood Cancer Journal |
| ISSN: | 2044-5385 |
| DOI: | 10.1038/bcj.2014.69 |
| Popis: | Multiparameter flow cytometry (MFC) and allele-specific oligonucleotide real-time quantitative PCR (ASO RQ-PCR) are the two most sensitive methods to detect minimal residual disease (MRD) in multiple myeloma (MM). We compared these methods in 129 paired post-therapy samples from 22 unselected, consecutive MM patients in complete/near complete remission. Appropriate immunophenotypic and ASO RQ-PCR-MRD targets could be detected and MRD analyses constructed for all patients. The high PCR coverage could be achieved by gradual widening of the primer sets used for clonality detection. In addition, for 13 (55%) of the patients, reverse orientation of the ASO primer and individual design of the TaqMan probe improved the sensitivity and specificity of ASO RQ-PCR analysis. A significant nonlinear correlation prevailed between MFC-MRD and PCR-MRD when both were positive. Discordance between the methods was found in 32 (35%) paired samples, which were negative by MFC-MRD, but positive by ASO RQ-PCR. The findings suggest that with the described technique, ASO RQ-PCR can be constructed for all patients with MM. ASO RQ-PCR is slightly more sensitive in MRD detection than 6−10-color flow cytometry. Owing to technical demands ASO RQ-PCR could be reserved for patients in immunophenotypic remission, especially in efficacy comparisons between different drugs and treatment modalities. |
| Databáze: | OpenAIRE |
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