Molecular Screening of Behaviorally Active Compounds with CmedOBP14 from the Rice Leaf Folder Cnaphalocrocis medinalis
| Autor: | Shan-Cheng Yi, Man-Qun Wang, Shuang-Feng Sun, Fang-Fang Zeng |
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| Rok vydání: | 2019 |
| Předmět: |
Arthropod Antennae
0106 biological sciences Chemoreceptor Odorant binding Olfaction Moths Receptors Odorant Binding Competitive 01 natural sciences Biochemistry chemistry.chemical_compound RNA interference Animals Ecology Evolution Behavior and Systematics RNA Double-Stranded Nerolidol Gene knockdown Behavior Animal biology General Medicine Hydrogen-Ion Concentration biology.organism_classification Affinities Cnaphalocrocis medinalis Electrophysiological Phenomena 010602 entomology chemistry Insect Proteins RNA Interference Norisoprenoids Sesquiterpenes 010606 plant biology & botany |
| Zdroj: | Journal of Chemical Ecology. 45:849-857 |
| ISSN: | 1573-1561 0098-0331 |
| DOI: | 10.1007/s10886-019-01106-z |
| Popis: | Odorant binding proteins (OBPs) play a key role in chemoreception in insects. In an earlier study, we identified CmedOBP14 from the rice leaf folder, Cnaphalocrocis medinalis, with potential physiological functions in olfaction. Here, we performed a competitive binding assay under different pH conditions as well as knockdown via RNA interference to determine the specific role of CmedOBP14 in C. medinalis. CmedOBP14 displayed broad binding affinities to many host-related compounds, with higher affinities at pH 7.4 compared with pH 5.0. After treatment with CmedOBP14-dsRNA, the transcript level of OBP14 was significantly decreased at 72 h compared with controls, and the electroantennogram response evoked by nerolidol, L-limonene and beta-ionone was reduced. Furthermore, behavioral assays revealed consistent patterns among these compounds, especially for nerolidol, with adults could no longer able to differentiate 0.1% nerolidol from controls. RNAi experiments suggest that at least in part, CmedOBP14 mediates the ability to smell nerolidol and beta-ionone. |
| Databáze: | OpenAIRE |
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