Human peripheral blood mononuclear cell in vitro system to test the efficacy of food bioactive compounds: Effects of polyunsaturated fatty acids and their relation with BMI
| Autor: | Andreu Palou, Jordi Pericás-Beltrán, Rubén Díaz-Rúa, Rubén Varela-Calviño, Paula Oliver, Bàrbara Reynés, Margalida Cifre |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Docosahexaenoic Acids Population Inflammation In Vitro Techniques Biology Polymerase Chain Reaction Peripheral blood mononuclear cell Body Mass Index 03 medical and health sciences Internal medicine Fatty Acids Omega-3 medicine Humans Obesity education chemistry.chemical_classification education.field_of_study 030109 nutrition & dietetics Dose-Response Relationship Drug Fatty Acids Lipid metabolism Lipid Metabolism Eicosapentaenoic acid Endocrinology Eicosapentaenoic Acid chemistry Docosahexaenoic acid Immunology Fatty Acids Unsaturated Leukocytes Mononuclear Cytokines medicine.symptom Ex vivo Food Science Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Molecular Nutrition & Food Research. 61:1600353 |
| ISSN: | 1613-4125 |
| DOI: | 10.1002/mnfr.201600353 |
| Popis: | cope : To analyse usefulness of isolated human peripheral blood mononuclear cells (PBMC) to rapidly/easily reflect n-3 long-chain polyunsaturated fatty acid (LCPUFA) effects on lipid metabolism/inflammation gene profile, and evaluate if these effects are BMI-dependent. Methods and results : PBMC from normoweight (NW) and overweight/obese (OW/OB) subjects were incubated with physiological doses of docosahexaenoic (DHA), eicosapentaenoic acid (EPA), or their combination. PBMC reflected increased beta-oxidation-like capacity (CPT1A expression) in OW/OB but only after DHA treatment. However, insensitivity to n-3 LCPUFA was evident in OW/OB for lipogenic genes: both PUFA diminished FASN and SREBP1C expression in NW, but no effect was observed for DHA in PBMC from high-BMI subjects. This insensitivity was also evident for inflammation gene profile: all treatments inhibited key inflammatory genes in NW; nevertheless, no effect was observed in OW/OB after DHA treatment, and EPA effect was impaired. SLC27A2, IL6 and TNFα PBMC expression analysis resulted especially interesting to determine obesity-related n-3 LCPUFA insensitivity. Conclusion : A PBMC-based human in vitro system reflects n-3 LCPUFA effects on lipid metabolism/inflammation which is impaired in OW/OB. These results confirm the utility of PBMC ex vivo systems for bioactive-compound screening to promote functional food development and to establish appropriate dietary strategies for obese population. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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