Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea
| Autor: | Thomas Edwards, Helen B. Smalley, Liz Gillies, Glyn Hobbs, Patricia A. Burke |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Time Factors Pcr assay Loop-mediated isothermal amplification Urine Biology medicine.disease_cause Sensitivity and Specificity Gonorrhea chemistry.chemical_compound Glutamate-Ammonia Ligase medicine Humans Urea Enzyme Inhibitors Temperature Bacteriology Nucleic acid amplification technique Molecular biology Neisseria gonorrhoeae QR Open reading frame Glutamine synthetase gene chemistry QR180 Nucleic Acid Amplification Techniques |
| Zdroj: | Journal of Clinical Microbiology. 52:2163-2165 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00314-14 |
| Popis: | A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of |
| Databáze: | OpenAIRE |
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