Effects of celecoxib on cycle kinetics of gastric cancer cells and protein expression of cytochrome C and caspase-9
Autor: | Xiao-Ping Niu, Li Yang, Zhen Han, Yu-Jie Wang, Ying-Jie Ma |
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Rok vydání: | 2013 |
Předmět: |
Cancer Research
Cell cycle checkpoint Epidemiology Blotting Western Apoptosis Biology Stomach Neoplasms Tumor Cells Cultured Humans Cell Proliferation Sulfonamides Cyclooxygenase 2 Inhibitors Cell growth Cytochrome c Cell Cycle Public Health Environmental and Occupational Health Cytochromes c Cell cycle Flow Cytometry Molecular biology Caspase 9 Cell biology Kinetics Oncology Celecoxib Cancer cell Cell Cycle Kinetics biology.protein Pyrazoles G1 phase |
Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 14(4) |
ISSN: | 2476-762X |
Popis: | Objective: This investigation aimed to determine effects of celecoxib on the cell cycle kinetics of the gastric cancer cell line MGC803 and the mechanisms involved by assessing expression of cytochrome C and caspase-9 at the protein level. Methods: Cell proliferation of MGC803 was determined by MTT assay after treatment with celecoxib. Apoptosis was assessed using fluorescence staining and cell cycle kinetics by flow cytometry. Western blotting was used to detect the expression of caspase-9 protein and of cytochrome C protein in cell cytosol and mitochondria. Results: Celecoxib was able to restrain proliferation and induce apoptosis in a dose- and timedependent manner, inducing G0/G1 cell cycle arrest, release of cytochrome C into the cytosol, and cleavage of pro-caspase-9 into its active form. Conclusion: Celecoxib can induce apoptosis in MGC803 cells through a mechanism involving cell cycle arrest, mitochondrial cytochrome C release and caspase activation. |
Databáze: | OpenAIRE |
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