N-Acetylcysteine and Benfotiamine Protect Autotransplanted Ovarian Tissue From Ischemia-Reperfusion Injury: An Experimental Study
| Autor: | Gokhan Artas, Şehmus Pala, Suleyman Aydin, Sevim Tuncer, Tuncay Kuloglu, Remzi Atilgan |
|---|---|
| Rok vydání: | 2023 |
| Předmět: |
0301 basic medicine
Transplantation medicine.medical_specialty 030219 obstetrics & reproductive medicine business.industry Ischemia medicine.disease Neovascularization Vascular endothelial growth factor Acetylcysteine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Benfotiamine Endocrinology chemistry Fibrosis Internal medicine medicine Folliculogenesis medicine.symptom business Reperfusion injury medicine.drug |
| Zdroj: | Experimental and Clinical Transplantation. 21:251-258 |
| ISSN: | 2146-8427 1304-0855 |
| DOI: | 10.6002/ect.2017.0320 |
| Popis: | Objectives This study aimed to compare the effects of N-acetylcysteine and benfotiamine in protection of ovarian tissue from ischemia caused by slow neovascularization injury due to intraperitoneal ovarian autotransplant in rats. Materials and methods Twenty-eight female rats were divided into 4 groups, each containing 7 rats. Group 1 only had the abdomen opened and closed, group 2 was the transplant-only group, group 3 received benfotiamine for 3 weeks starting 1 day before the transplant procedure, and group 4 received N-acetylcysteine for 3 weeks starting 1 day before the transplant procedure. At the end of the experimental period, malondialdehyde levels in ovarian tissues together with the apoptosis and fibrosis, proliferating cell nuclear antigen and vascular endothelial growth factor immunoreactivity, and ovarian reserves were investigated. Results Apoptosis was significantly increased in group 2 animals. Primordial follicle count was higher in groups 3 and 4 than in group 2. Vascular endothelial growth factor immunoreactivity was decreased in groups 3 and 4 compared with group 2. Proliferating cell nuclear antigen immunoreactivity was reduced in the secondary follicles in all transplant groups. Conclusions In autologous intraperitoneal ovarian transplant, both benfotiamine and N-acetylcysteine are equal and effective agents in protection of ovarian tissue against ischemic injury. |
| Databáze: | OpenAIRE |
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