Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population
| Autor: | Héctor Flores-Herrera, Omar Martínez-Alarcón, Néstor F. Díaz, Juan Carlos Regalado-Hernández, Anayansi Molina-Hernández, Eva Vega-Hernández, Julio Francisco De la Jara-Díaz, Guadalupe García-López, Guadalupe Razo-Aguilera, Néstor E. Díaz-Martínez, María Yolotzin Valdespino-Vázquez, Wendy Portillo, Elsa Romelia Moreno-Verduzco, Daniela Ávila-González |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Population Human Embryonic Stem Cells Cell Culture Techniques Biology Cell Line Andrology 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Humans education lcsh:QH301-705.5 Mexico education.field_of_study Karyotype Embryo Cell Biology General Medicine medicine.disease Embryo Mammalian Embryonic stem cell Mexican population 030104 developmental biology lcsh:Biology (General) Cell culture embryonic structures Female Teratoma 030217 neurology & neurosurgery Developmental Biology Human embryonic stem cell line |
| Zdroj: | Stem Cell Research, Vol 34, Iss, Pp-(2019) |
| ISSN: | 1876-7753 |
| Popis: | Although investigation with human embryonic stem cells (HESC) is not decreasing, the derivation of new lines has been diminished. The preeminence of only a few HESC lines in research is accompanied by lack of universal applicability of results as well as by genetic under-representation. We previously reported the derivation of one line with male karyotype from Mexican population. Here, we derived one HESC line (Amicqui-2) with female karyotype from poor-quality embryos. These line comply the pluripotent requirements (normal karyotype, detection of pluripotency-associated markers, mycoplasma test and teratoma formation) and could be a valuable model for studying diseases specific to under-represented population. |
| Databáze: | OpenAIRE |
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