COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells
| Autor: | Mark Leid, Sylvette Chasserot-Golaz, Olivier Rohr, Laetitia Redel, Céline Marban, Stella Suzanne, Evelyne Schaeffer, Dominique Lecestre, Dominique Aunis, Carine Van Lint |
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| Přispěvatelé: | Institut de Recherche sur les Maladies Virales et Hépatiques (IVH), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Immunopathologie et chimie thérapeutique (ICT), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Dynamique des interactions Hôte pathogène, Université de Strasbourg (UNISTRA), P30 ES000210, Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | francouzština |
| Rok vydání: | 2005 |
| Předmět: |
Receptors
Steroid Repressor Proteins -- analysis Transcription Genetic analysis physiology Chromosomal Proteins Non-Histone Response element DNA-Binding Proteins -- physiology Virus Replication Receptors Steroid -- antagonists & inhibitors 0302 clinical medicine Sciences du Vivant [q-bio]/Biologie cellulaire genetics Chromosomal Proteins Non-Histone -- analysis antagonists & inhibitors chemistry 0303 health sciences General transcription factor Nuclear Proteins DNA-Binding Proteins -- analysis TCF4 Sciences bio-médicales et agricoles 3. Good health DNA-Binding Proteins 030220 oncology & carcinogenesis TAF2 Cell Nucleus Structures -- chemistry Microglia Sp1 Transcription Factor -- antagonists & inhibitors Carrier Proteins -- physiology Transcription Factors -- antagonists & inhibitors Gene Expression Regulation Viral Sp1 Transcription Factor Repressor Proteins -- physiology E-box DNA-Binding Proteins -- antagonists & inhibitors [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Article Cell Line 03 medical and health sciences HIV-1 -- physiology Sp3 transcription factor HIV-1 -- genetics Genetics Humans Enhancer Sp1 Transcription Factor -- chemistry 030304 developmental biology HIV Long Terminal Repeat Tumor Suppressor Proteins Promoter DNA-Binding Proteins -- chemistry Microglia -- virology Molecular biology Transcription Factors -- analysis Cell Nucleus Structures Protein Structure Tertiary Repressor Proteins Transcription Factors -- chemistry COUP Transcription Factors Nuclear Proteins -- physiology Receptors Steroid -- analysis Chromobox Protein Homolog 5 metabolism virology HIV-1 Microglia -- metabolism Carrier Proteins Receptors Steroid -- chemistry Transcription Factors Sp1 Transcription Factor -- analysis |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2005, 33 (7), ⟨10.1093/nar/gki529⟩ Nucleic acids research, 33 (7 |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gki529⟩ |
| Popis: | Human immunodeficiency virus type 1 (HIV-1) gene transcription is characterized by two temporally distinct phases. While the initial phase relies solely on cellular transcription factors, the subsequent phase is activated by the viral Tat transactivator. We have previously reported that the subsequent phase of viral gene transcription can be repressed by the chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting protein 2 (CTIP2) in human microglial cells [O. Rohr, D. Lecestre, S. Chasserot-Golaz, C. Marban, D. Avram, D. Aunis, M. Leid and E. Schaeffer (2003), J. Virol. 77, 5415-5427]. Here, we demonstrate that CTIP proteins also repress the initial phase of HIV-1 gene transcription, mainly supported by the cellular transcription factors Sp1 and COUP-TF in microglial cells. We report that CTIP2 represses Sp1- and COUP-TF-mediated activation of HIV-1 gene transcription and viral replication as a result of physical interactions with COUP-TF and Sp1 in microglial nuclei. Using laser confocal microscopy CTIP2 was found to colocalize with Sp1, COUP-TF and the heterochromatin-associated protein Hp1alpha, which is mainly detected in transcriptionally repressed heterochromatic region. Moreover, we describe that CTIP2 can be recruited to the HIV-1 promoter via its association with Sp1 bound to the GC-box sequences of the long terminal repeat (LTR). Since our findings demonstrate that CTIP2 interacts with the HIV-1 proximal promoter, it is likely that CTIP2 promotes HIV-1 gene silencing by forcing transcriptionally repressed heterochromatic environment to the viral LTR region. Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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