Established UPLC-MS/MS procedure for multicomponent quantitative analysis in rat plasma: A contrastive pharmacokinetics study of Qiangshen tablet in normal and kidney yang deficiency syndrome models
| Autor: | Pan-Pan Xu, Jinmei Chen, Guang-Hui Gao, Lixin Sun, Binglong Li, Jinbo Yu, Jinwei Gao |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Proline Formic acid Clinical Biochemistry Ethyl acetate 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Glucosides Tandem Mass Spectrometry Furocoumarins Protein precipitation Animals Iridoids Glycosides Chromatography High Pressure Liquid Chromatography Loganin 010401 analytical chemistry Selected reaction monitoring Ficusin Cell Biology General Medicine Paeoniflorin 0104 chemical sciences Rats Yang Deficiency chemistry Metabolome Monoterpenes Drug Monitoring Quantitative analysis (chemistry) Drugs Chinese Herbal Tablets |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. |
| ISSN: | 1873-376X |
| Popis: | Qiangshen tablet, an important prescription consisting of 14 kinds of Chinese herbal medicines, has been used for decades to treat kidney yang deficiency syndrome (KYDS) in China. Qiangshen tablet has been recorded in ChP (2015 edition) and possesses the effect of strengthening yang, invigorating qi and tonifying kidneys. In this research, a simple, reliable and specific method was established for simultaneous determination of stachydrine, psoralen, isopsoralen, morroniside, paeoniflorin and loganin in normal and KYDS rat plasma after intragastric administration of a Qiangshen tablet suspension by UPLC-MS/MS. Protein precipitation (PP) by acetonitrile and liquid-liquid extraction (LLE) by ethyl acetate - n-butanol (1: 1, v/v) were used for pretreatment of plasma samples. Chromatographic separation of two IS (Internal Standard) and six analytes was achieved using an ACQUITY UPLC® BEH C18 column (2.1 × 100 mm, 1.7 μm). The mobile phase consisted of 0.1% formic acid aqueous solution (solvent A) and acetonitrile (solvent B) with a gradient scheme. Multiple reaction monitoring (MRM) mode with positive and negative ion source switching was applied to perform the mass spectrometric analyses. This method has been validated with good linearity (r ≥ 0.9942) and acceptable precision and accuracy (RSD ≤ 11%, RE from −4.8% to 7.7%). The mean recovery values of the analytes and IS were all ≥68.28%, and the matrix effects ranged from 94.4% to 101.7%. The stability of the IS and analytes was measured throughout the experiment. The results showed significant differences between the pharmacokinetic traits of the analytes in the normal and KYDS groups, suggesting that pharmacokinetic procedures involving these analytes could be modified in cases of KYDS. |
| Databáze: | OpenAIRE |
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