An immunohistochemical study of the distribution of biotin in tissues of pigs and chickens
| Autor: | S Kennedy, S. McConnell, D.G. Kennedy, M Frigg, Kevin M. Cooper |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Streptavidin Pathology medicine.medical_specialty Swine Biotin Adipose tissue Biology chemistry.chemical_compound medicine Animals Tissue Distribution Microscopy Confocal General Veterinary Immunohistochemistry Primary and secondary antibodies Epithelium medicine.anatomical_structure Liver chemistry Organ Specificity Dietary Supplements biology.protein Chickens Immunostaining Avidin |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/s0034-5288(97)90024-2 |
| Popis: | An optimised indirect peroxidase-anti-peroxidase immunohistochemical technique was used to detect endogenous biotin in frozen tissue sections from biotin-supplemented and biotin-depleted pigs and chickens. A monoclonal anti-biotin antibody was used as primary antibody in this technique. Immunoreactive biotin was detected in many tissues of both species including liver, kidney, pancreas, adipose tissue, adrenal gland, testis, brain, choroid plexus, cardiac and skeletal muscle, epithelium of the respiratory and digestive systems, skin and lymphoid tissues. The specificity of immunostaining for biotin was confirmed by the finding of reduced staining intensities in tissues of biotin-depleted animals compared to those of biotin-supplemented animals. The results of this study suggest that biotin has metabolic functions in a wider range of tissues than previously known. They also indicate that endogenous tissue biotin should be considered as a source of false-positive staining when immunohistochemical or histochemical techniques which use avidin or streptavidin reagents or anti-biotin antibodies as components of the detection system, are applied to tissue sections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect