New Insights into the Potential Cytotoxic Role of Bacillus cytotoxicus Cytotoxin K-1
| Autor: | Andreja Rajkovic, Jacques Mahillon, Jelena Jovanovic, Pauline Hinnekens, Klèma Marcel Koné |
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| Jazyk: | angličtina |
| Předmět: |
Agriculture and Food Sciences
Technology and Engineering GENES Health Toxicology and Mutagenesis Mutant Bacillus cereus DIVERSITY Bacillus cytotoxicus medicine.disease_cause Toxicology TOXICITY Microbiology CytK-1 03 medical and health sciences THURINGIENSIS medicine Cytotoxic T cell Toxicology and Mutagenesis Cytotoxicity Gene 030304 developmental biology 0303 health sciences CEREUS biology FUNCTIONAL-ANALYSIS 030306 microbiology Toxin RANGE STRAINS Biology and Life Sciences Caco-2 biology.organism_classification 3. Good health Complementation Cell culture Health Medicine cytotoxicity |
| Zdroj: | TOXINS Toxins, Vol 13, Iss 698, p 698 (2021) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins13100698 |
| Popis: | The thermotolerant representative of the Bacillus cereus group, Bacillus cytotoxicus, reliably harbors the coding gene of cytotoxin K-1 (CytK-1). This protein is a highly cytotoxic variant of CytK toxin, initially recovered from a diarrheal foodborne outbreak that caused the death of three people. In recent years, the cytotoxicity of B. cytotoxicus has become controversial, with some strains displaying a high cytotoxicity while others show no cytotoxicity towards cell lines. In order to better circumscribe the potential pathogenic role of CytK-1, knockout (KO) mutants were constructed in two B. cytotoxicus strains, E8.1 and E28.3. The complementation of the cytK-1 KO mutation was implemented in a mutant strain lacking in the cytK-1 gene. Using the tetrazolium salt (MTT) method, cytotoxicity tests of the cytK-1 KO and complemented mutants, as well as those of their wild-type strains, were carried out on Caco-2 cells. The results showed that cytK-1 KO mutants were significantly less cytotoxic than the parental wild-type strains. However, the complemented mutant was as cytotoxic as the wild-type, suggesting that CytK-1 is the major cytotoxicity factor in B. cytotoxicus. |
| Databáze: | OpenAIRE |
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