Some microbiological aspects of radiation sterilization
| Autor: | George J. Fuld, Bernard E. Proctor, Samuel A. Goldblith |
|---|---|
| Rok vydání: | 1957 |
| Předmět: |
Sodium ascorbate
Radiation Bacteria biology Clostridium sporogenes medicine.medical_treatment Sterilization Bacillus subtilis Sterilization (microbiology) biology.organism_classification medicine.disease_cause Microbiology Radiation Effects chemistry.chemical_compound Nuclear Energy and Engineering chemistry medicine Radiology Nuclear Medicine and imaging Radiosensitivity Food science Escherichia coli Saline |
| Zdroj: | The International Journal of Applied Radiation and Isotopes. 2:35-43 |
| ISSN: | 0020-708X |
| DOI: | 10.1016/0020-708x(57)90024-8 |
| Popis: | Pure suspensions of bacteria, Escherichia coli, Bacillus subtilis, B. thermoacidurans, and several putrefactive anaerobes denoted as PA #76H and PA #3679 (species related to Clostridium sporogenes), were irradiated both by cathode rays and by gamma rays from cobalt-60. These studies emphasize that broad generalizations are not possible on the effects of different environments on the radiosensitivity of a bacterial population. When a free-radical acceptor such as sodium ascorbate was added to saline suspensions of B. subtilis, the radioresistance of the organism was increased, but when sodium ascorbate was added to saline suspensions of PA #76H, PA #3679, and B. thermoacidurans, the radioresistances of these organisms were no different from those when they were irradiated in saline solution alone. All these organisms (B. subtilis, B. thermoacidurans, PA #76H, and PA #3679) were more radioresistant when irradiated in the presence of sodium ascorbate in more complex media such as pea puree and tomato juice. Comparisons of the radioresistances of these organisms in saline solution and in a more complex medium also show differences. B. subtilis was less radioresistant, whereas B. thermoacidurans, PA #76H, and PA #3679 were more radioresistant in a more complex medium than in a saline solution. The effects of pH, the atmosphere during irradiation, the type of radiation, and the state of the medium on the radiosensitivities of these organisms are discussed. Recent data on the putrefactive anaerobes are presented to illustrate the handling of data and the statistical method utilized. Considerations are given for examining heterogeneous populations of bacteria encountered in various foods to be irradiated. |
| Databáze: | OpenAIRE |
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