Knockdown of VEGFR2 Inhibits Proliferation and Induces Apoptosis in Hemangioma-Derived Endothelial Cells
| Autor: | Ou, J.M., Yu, Z.Y., Qiu, M.K., Dai, Y.X., Dong, Q., Shen, J., Dong, P., Wang, X.F., Liu, Y.B., Quan, Z.W., Fei, Z.W. |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
vascular endothelial growth factor receptor 2
hemangioma proliferation apoptosis Histology Angiogenesis proliferation Biophysics Biology Small hairpin RNA chemistry.chemical_compound vascular endothelial growth factor receptor 2 Cell Line Tumor Humans Viability assay Extracellular Signal-Regulated MAP Kinases lcsh:QH301-705.5 Protein kinase B Cell Proliferation Gene knockdown Original Paper Cell growth Caspase 3 apoptosis Endothelial Cells Kinase insert domain receptor Cell Biology respiratory system Vascular Endothelial Growth Factor Receptor-2 Cell biology Vascular endothelial growth factor Gene Expression Regulation Neoplastic hemangioma Ki-67 Antigen lcsh:Biology (General) chemistry Gene Knockdown Techniques cardiovascular system Proto-Oncogene Proteins c-akt circulatory and respiratory physiology |
| Zdroj: | European Journal of Histochemistry : EJH European Journal of Histochemistry, Vol 58, Iss 1 (2014) |
| ISSN: | 2038-8306 1121-760X |
| Popis: | Angiogenesis is a process of development and growth of new capillary blood vessels from pre-existing vessels. Angiogenic growth factors play important roles in the development and maintenance of some malignancies, of which vascular endothelial growth factor (VEGF)/VEGFR2 interactions are involved in proliferation, migration, and survival of many cancer cells. The aim of this study was to investigate the function of VEGFR2 in human hemangiomas (HAs). Using immunohistochemistry assay, we examined the expression levels of VEGF, VEGFR2, Ki-67, glucose transporter-1 (Glut-1), phosphorylated protein kinase B (p-AKT) and p-ERK in different phases of human HAs. Positive expression of VEGF, VEGFR2, Ki-67, Glut-1, p-AKT and p-ERK was significantly increased in proliferating phase HAs, while decreased in involuting phase HAs (P=0.001; P=0.003). In contrast, cell apoptotic indexes were decreased in proliferating phase HAs, but increased in involuting phase HAs (P |
| Databáze: | OpenAIRE |
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