Preclinical evaluation of group B Neisseria meningitidis and Escherichia coli K92 capsular polysaccharide-protein conjugate vaccines in juvenile rhesus monkeys
| Autor: | F. Norelli, J. Y. Tai, W D Zollinger, Rino Rappuoli, P. J. Snoy, Carl E. Frasch, P. Costantini, S. J. N. Devi |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Immunology Neisseria meningitidis medicine.disease_cause Microbiology Antigen Conjugate vaccine Escherichia coli Tetanus Toxoid medicine Animals Humans Bacterial Capsules Vaccines Conjugate biology Polysaccharides Bacterial Toxoid Antibodies Bacterial Macaca mulatta Virology Bacterial vaccine Infectious Diseases Immunoglobulin M Immunoglobulin G Bacterial Vaccines biology.protein Female Immunization Parasitology Antibody Bacterial Outer Membrane Proteins Research Article Conjugate |
| Zdroj: | Infection and Immunity. 65:1045-1052 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | We reported the first use of group B meningococcal conjugate vaccines in a nonhuman primate model (S. J. N. Devi, C. E. Frasch, W. Zollinger, and P. J. Snoy, p. 427-429, in J. S. Evans, S. E. Yost, M. C. J. Maiden, and I. M. Feavers, ed., Proceedings of the Ninth International Pathogenic Neisseria Conference, 1994). Three different group B Neisseria meningitidis capsular polysaccharide (B PS)-protein conjugate vaccines and an Escherichia coli K92 capsular polysaccharide-tetanus toxoid (K92-TT) conjugate vaccine are here evaluated for safety and relative immunogenicities in juvenile rhesus monkeys with or without adjuvants. Monkeys were immunized intramuscularly with either B PS-cross-reactive material 197 conjugate, B PS-outer membrane vesicle (B-OMV) conjugate, or N-propionylated B PS-outer membrane protein 3 (N-pr. B-OMP3) conjugate vaccine with or without adjuvants at weeks 0, 6, and 14. A control group of monkeys received one injection of the purified B PS alone, and another group received three injections of B PS noncovalently complexed with OMV. Antibody responses as measured by enzyme-linked immunosorbent assay varied among individual monkeys. All vaccines except B PS and the K92-TT conjugate elicited a twofold or greater increase in total B PS antibodies after one immunization. All vaccines, including the K92-TT conjugate, elicited a rise in geometric mean B PS antibody levels of ninefold or more over the preimmune levels following the third immunization. Antibodies elicited by N-pr. B-OMP3 and B-OMV conjugates were directed to the N-propionylated or to the spacer-containing B PS antigens as well as to the native B PS complexed with methylated human serum albumin. None of the vaccines caused discernible safety-related symptoms. |
| Databáze: | OpenAIRE |
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