Olmesartan decreases IL-1β and TNF-α levels; downregulates MMP-2, MMP-9, COX-2, and RANKL; and upregulates OPG in experimental periodontitis
| Autor: | Karoline S. Aragão, Graziene Lopes de Souza, Maria do Socorro Costa Feitosa Alves, Raimundo Fernandes de Araújo, Yriu Lourenço, Tatiana Oliveira Souza, Aurigena Antunes de Araújo, Gerly Anne de Castro Brito, Caroline Addison Carvalho Xavier de Medeiros |
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| Jazyk: | portugalština |
| Rok vydání: | 2013 |
| Předmět: |
musculoskeletal diseases
Male medicine.medical_specialty Interleukin-1beta Alveolar Bone Loss Anti-Inflammatory Agents Gingiva Tetrazoles Osteoprotegerin Internal medicine Malondialdehyde medicine Animals Immunohistochemical Olmesartan Rats Wistar Lipid peroxidant Receptor Periodontitis Peroxidase Pharmacology biology Receptor Activator of Nuclear Factor-kappa B Chemistry Tumor Necrosis Factor-alpha RANK Ligand Imidazoles Interleukin General Medicine Angiotensin II Rats Endocrinology Matrix Metalloproteinase 9 Experimental periodontal disease RANKL Cyclooxygenase 2 Myeloperoxidase Citocines biology.protein Matrix Metalloproteinase 2 Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
| Popis: | The objective of this study is to investigate the participation of inflammatory and oxidative stress mediators and the effects on the expression of matrix metalloproteinase (MMP)-2, MMP-9, and receptor activator of NF-κB ligand (RANKL)/receptor activator of NF-κB (RANK)/osteoprotegerin (OPG) pathway in the response to treatment with olmesartan, an angiotensin II type 1 receptor blocker. Male Wistar albino rats were randomly divided into five groups of ten rats each: (1) non-ligature with water, (2) ligature with water, (3) ligature with 1 mg/kg olmesartan, (4) ligature with 6 mg/kg olmesartan, and (5) ligature with 10 mg/kg olmesartan. All groups were treated with olmesartan or the vehicle by gavage daily for 10 days. Following the treatment course, the periodontal tissue of the animals was analyzed by histopathology and immunohistochemistry to determine the expression of cyclooxygenase-2 (COX-2), MMP-2, MMP-9, and members of the RANKL/RANK/OPG pathway and by ELISA and spectroscopic assay to determine the levels of interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), malonaldehyde (MDA), and glutathione. The concentrations of MPO and MDA were reduced in the group that received 6 mg/kg olmesartan (p |
| Databáze: | OpenAIRE |
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