mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo
Autor: | Carol Katayama, Hugo K. Dooner, Caroline Dean, John Bedbrook, Pamela Dunsmuir, Stanley Tamaki, Mitchell Favreau |
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Rok vydání: | 1986 |
Předmět: |
Genetics
Chlorophyll Messenger RNA Polyadenylation Base Sequence Sequence analysis Protein subunit Ribulose-Bisphosphate Carboxylase Biology Plants Molecular biology Genes Transcription (biology) Complementary DNA Consensus sequence RNA Messenger RNA Processing Post-Transcriptional Carrier Proteins Poly A Gene Plant Proteins |
Zdroj: | Nucleic acids research. 14(5) |
ISSN: | 0305-1048 |
Popis: | We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence. |
Databáze: | OpenAIRE |
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