mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo

Autor: Carol Katayama, Hugo K. Dooner, Caroline Dean, John Bedbrook, Pamela Dunsmuir, Stanley Tamaki, Mitchell Favreau
Rok vydání: 1986
Předmět:
Zdroj: Nucleic acids research. 14(5)
ISSN: 0305-1048
Popis: We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence.
Databáze: OpenAIRE