Differential effects of carbohydrates on arabidopsis pollen germination
| Autor: | Dominik K. Großkinsky, José M. García Fernández, Edith Stabentheiner, Jörg Hirsche, Thomas Roitsch |
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| Přispěvatelé: | Junta de Andalucía, Bavarian Ministry of the Environment and Consumer Protection, German Research Foundation, Ministry of Education, Youth and Sports (Czech Republic), Danish Council for Independent Research, Ministry of Higher Education and Science (Denmark), European Commission |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Arabidopsis thaliana Physiology Arabidopsis Carbohydrates Mannose Oligosaccharides Germination Plant Science medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Pollen Metabolic regulation Botany medicine Sugar Hexoses biology Chemistry Structure-function relationship Plant physiology food and beverages Cell Biology General Medicine biology.organism_classification Signaling 030104 developmental biology Pollen germination Carbohydrate Metabolism Energy source 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname idUS. Depósito de Investigación de la Universidad de Sevilla idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) |
| Popis: | Pollen germination as a crucial process in plant development strongly depends on the accessibility of carbon as energy source. Carbohydrates, however, function not only as a primary energy source, but also as important signaling components. In a comprehensive study, we analyzed various aspects of the impact of 32 different sugars on in vitro germination of Arabidopsis pollen comprising about 150 variations of individual sugars and combinations. Twenty-six structurally different mono-, di- and oligosaccharides, and sugar analogs were initially tested for their ability to support pollen germination. Whereas several di- and oligosaccharides supported pollen germination, hexoses such as glucose, fructose and mannose did not support and even considerably inhibited pollen germination when added to germination-supporting medium. Complementary experiments using glucose analogs with varying functional features, the hexokinase inhibitor mannoheptulose and the glucose-insensitive hexokinase-deficient Arabidopsis mutant gin2-1 suggested that mannose- and glucose-mediated inhibition of sucrose-supported pollen germination depends partially on hexokinase signaling. The results suggest that, in addition to their role as energy source, sugars act as signaling molecules differentially regulating the complex process of pollen germination depending on their structural properties. Thus, a sugar-dependent multilayer regulation of Arabidopsis pollen germination is supported, which makes this approach a valuable experimental system for future studies addressing sugar sensing and signaling. This study was supported by the Bayerisches Staatsministerium für Umwelt, Gesundheit und Verbraucherschutz, the SFB 56 and the DFG Graduiertenkolleg 1342 [to T.R.]; MINECO [contract No. CTQ2015-64425-C2-1-R to J.M.G.F]; the Junta de Andalucía [contract No. FQM2012-1467 to J.M.G.F]; the European Regional Development Funds (FEDER) [to J.M.G.F.]; the Ministry of Education, Youth and Sports of CR within the National Sustainability Program I (NPU I) [grant No. LO1415 to T.R.]; and by the Danish Council for Independent Research, Danish Ministry of Higher Education and Science to [Individual Postdoctoral Grant No. 4093-00255 to D.K.G.]. |
| Databáze: | OpenAIRE |
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