Production of lipase from Geotrichum sp and adsorption studies on affinity resin
| Autor: | Eliana Setsuko Kamimura, O. Mendieta, Hélia Harumi Sato, Francisco Maugeri, Glaucia Maria Pastore |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chromatography
biology General Chemical Engineering affinity resin kinetic constant lcsh:TP155-156 Industrial fermentation Sepharose Oleic acid chemistry.chemical_compound Adsorption chemistry Affinity chromatography adsorption Reagent lipase biology.protein Fermentation lcsh:Chemical engineering Lipase |
| Zdroj: | Brazilian Journal of Chemical Engineering v.16 n.2 1999 Brazilian Journal of Chemical Engineering Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ Brazilian Journal of Chemical Engineering, Vol 16, Iss 2, Pp 103-112 (1999) Brazilian Journal of Chemical Engineering, Volume: 16, Issue: 2, Pages: 103-112, Published: JUN 1999 |
| ISSN: | 0104-6632 |
| DOI: | 10.1590/s0104-66321999000200001 |
| Popis: | There is a growing interest in microbial lipase production due to its great potential for industrial applications such as food additives, industrial reagents and stain removers, as well as for medical applications. Specially for medical applications a high degree of purity is required, which is accomplished with high resolution chromatographic techniques. Affinity chromatography is considered a very high resolution chromatographic technique. In this work the adsorption isotherms and kinetics of the adsorption of lipase from Geotrichum sp on biospecific resin were determined. The resin was prepared using EAH sepharose 4B gel (Pharmacia), made to react with oleic acid as the specific ligand.The lipase was produced in a five-liter fermenter, with both complex and synthetic media. Fermentation conditions were a temperature of 30°C, an aeration of 1VVM and an agitation of 400 rpm. Maximum lipase activity was around 28 U/ml after 10 hours of fermentation for the complex medium. The kinetic model and parameters were determined by dynamic fitting to experimental results using the fourth-order Runge-Kutta method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|