Antibody against pulmonary surfactant protein A recognizes proteins in intestine and swim bladder of the freshwater fish, carp
| Autor: | Robert Ducroc, Bernadette Chailley-Heu, Sandrine Rubio, Jacques R. Bourbon |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gill
Carps Pulmonary Surfactant-Associated Proteins Alkylation Proteolipids Blotting Western Biophysics Biochemistry Antibodies Intestinal mucosa Species Specificity Swim bladder Animals Electrophoresis Gel Two-Dimensional Tissue Distribution Intestinal Mucosa Carp Molecular Biology Phylogeny biology Air Sacs Pulmonary Surfactant-Associated Protein A Proteins Pulmonary Surfactants Cell Biology biology.organism_classification Molecular biology Rats Blot biology.protein Freshwater fish Antibody Protein A |
| Zdroj: | Biochemical and biophysical research communications. 225(3) |
| ISSN: | 0006-291X |
| Popis: | An antibody raised against rat pulmonary surfactant protein A (SP-A) bound on Western blots to proteins present in intestinal mucosa and in swim bladder, but not in gills of the carp. The fish protein(s) revealed by the antibody exhibited an electrophoretical behavior similar to that of rat SP-A with a characteristic triplet in the 30- to 35-kDa range. It therefore appears that proteins immunologically very close to mammalian SP-A are present in modern fish which evolved from ancestors that never possessed lungs. The association of SP-A with phospholipid-rich surfactant-like materials appears as a phylogenetically old feature. |
| Databáze: | OpenAIRE |
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