| Autor: |
Hernán Ramiro Lascano, Pablo Javier Gonzalez, Mariana Melchiorre, Mauricio Javier Lozano, Antonio Lagares |
| Rok vydání: |
2018 |
| Předmět: |
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| DOI: |
10.1101/363556 |
| Popis: |
Drought and salinity conditions are the major factors affecting nitrogen fixation by legume-rhizobium symbiosis. A global response to these stress conditions is the increase of intracellular ROS leading to activation of antioxidant system to ensure cellular homeostasis. Under the hypothesis that overexpression ofsodgene inM. lotiimprove tolerance to oxidative stress we decide to investigate the response of constitutive overexpression of theMesorhizobium lotiMAFF303099sodgene on tolerance to oxidative stress under free-living conditions. The genemlr7636was overexpressed constitutively under thenptIIpromoter of pFAJ1708 plasmid. Our study revealed that thesodoverexpressing mutant had five-fold increased SOD activity in periplasmic space and a better tolerance to superoxide and hydrogen peroxide in bacterial killing assays.IMPORTANCEIn this study, we report that the homologous superoxide dismutase overexpression inMesorhizobium lotiimproves its tolerance to oxidative stress under free-living conditions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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