A systems approach to designing next generation vaccines: combining α-galactose modified antigens with nanoparticle platforms
| Autor: | Krishna Rajan, Robert Barry Mandell, Michael J. Wannemuehler, Yashdeep Phanse, Chang Sun Kong, Brenda R. Carrillo-Conde, Ramon Flick, Scott Broderick, Balaji Narasimhan, Amanda E. Ramer-Tait |
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| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
Systems biology medicine.medical_treatment media_common.quotation_subject Antigen-Presenting Cells Biology Article Epitope Epitopes Immune system Adjuvants Immunologic Antigen Immunity medicine Humans Antigens Antigen-presenting cell Internalization media_common Vaccines Multidisciplinary Systems Biology Virology Immunity Innate alpha-Galactosidase Nanoparticles Peptides Adjuvant |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep03775 |
| Popis: | Innovative vaccine platforms are needed to develop effective countermeasures against emerging and re-emerging diseases. These platforms should direct antigen internalization by antigen presenting cells and promote immunogenic responses. This work describes an innovative systems approach combining two novel platforms, αGalactose (αGal)-modification of antigens and amphiphilic polyanhydride nanoparticles as vaccine delivery vehicles, to rationally design vaccine formulations. Regimens comprising soluble αGal-modified antigen and nanoparticle-encapsulated unmodified antigen induced a high titer, high avidity antibody response with broader epitope recognition of antigenic peptides than other regimen. Proliferation of antigen-specific CD4(+) T cells was also enhanced compared to a traditional adjuvant. Combining the technology platforms and augmenting immune response studies with peptide arrays and informatics analysis provides a new paradigm for rational, systems-based design of next generation vaccine platforms against emerging and re-emerging pathogens. |
| Databáze: | OpenAIRE |
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