Impact of CCR1 silencing on the assembly of lignified secondary walls in Arabidopsis thaliana

Autor: Katia Ruel, Brigitte Pollet, Jimmy Berrio-Sierra, Johanne Thévenin, Jean-Paul Joseleau, Mohammad Mir Derikvand, Lise Jouanin, Catherine Lapierre
Přispěvatelé: Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), Chimie Biologique (UCB), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Marie-Curie Grant, Institut National de la Recherche Agronomique (INRA)-CEPIA Department, France
Jazyk: angličtina
Rok vydání: 2009
Předmět:
0106 biological sciences
Magnetic Resonance Spectroscopy
Physiology
laser capture microdissection
Arabidopsis
Flowers
Plant Science
Biology
cell wall ultrastructure
Polysaccharide
quantitative immunogold labeling
Lignin
01 natural sciences
Gas Chromatography-Mass Spectrometry
Cell wall
03 medical and health sciences
chemistry.chemical_compound
Immunolabeling
X-Ray Diffraction
Cell Wall
[SDV.IDA]Life Sciences [q-bio]/Food engineering
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Gene Silencing
Cellulose
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Xylose
Plant Stems
Staining and Labeling
Plant Extracts
fungi
Xylem
food and beverages
Vascular bundle
Aldehyde Oxidoreductases
Immunohistochemistry
Glucose
chemistry
Biochemistry
Mutation
Biophysics
Carbohydrate Metabolism
Cinnamoyl-CoA reductase
Microdissection
Electron Probe Microanalysis
010606 plant biology & botany
Zdroj: New Phytologist
New Phytologist, Wiley, 2009, 184 (1), pp.99-113. ⟨10.1111/j.1469-8137.2009.02951.x⟩
ISSN: 0028-646X
1469-8137
Popis: International audience; A cinnamoyl-CoA reductase 1 knockout mutant in Arabidopsis thaliana was investigated for the consequences of lignin synthesis perturbation on the assembly of the cell walls. The mutant displayed a dwarf phenotype and a strong collapse of its xylem vessels corresponding to lower lignin content and a loss of lignin units of the noncondensed type. Transmission electron microscopy revealed that the transformation considerably impaired the capacity of interfascicular fibers and vascular bundles to complete the assembly of cellulose microfibrils in the S(2) layer, the S(1) layer remaining unaltered. Such disorder in cellulose was correlated with X-ray diffraction showing altered organization. Semi-quantitative immunolabeling of lignins showed that the patterns of distribution were differentially affected in interfascicular fibers and vascular bundles, pointing to the importance of noncondensed lignin structures for the assembly of a coherent secondary wall. The use of laser capture microdissection combined with the microanalysis of lignins and polysaccharides allowed these polymers to be characterized into specific cell types. Wild-type A. thaliana displayed a two-fold higher syringyl to guaiacyl ratio in interfascicular fibers compared with vascular bundles, whereas this difference was less marked in the cinnamoyl-CoA reductase 1 knockout mutant.
Databáze: OpenAIRE
Externí odkaz: