COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting
| Autor: | Steven B. Bradfute, Ivy Hurwitz, Qiuying Cheng, Myissa Weiss, Alexandra Yingling, Christopher S Bartlett, Jens Langsjoen, Douglas J Perkins |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) Health Personnel viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) medicine.disease_cause General Biochemistry Genetics and Molecular Biology Specimen Handling Hospitals University 03 medical and health sciences Health personnel 0302 clinical medicine Pandemic medicine Humans 030212 general & internal medicine Pandemics Original Research Coronavirus SARS-CoV-2 Transmission (medicine) business.industry virus diseases COVID-19 Reproducibility of Results medicine.disease University hospital Highlight article: Translational Research Health Planning 030104 developmental biology Touch Fomites Spike Glycoprotein Coronavirus RNA Viral Medical emergency business |
| Zdroj: | Experimental Biology and Medicine |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/15353702211024597 |
| Popis: | As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has surged across the globe, great effort has been expended to understand mechanisms of transmission and spread. From a hospital perspective, this topic is critical to limit and prevent SARS-CoV-2 iatrogenic transmission within the healthcare environment. Currently, the virus is believed to be transmitted primarily through respiratory droplets, but a growing body of evidence suggests that spread is also possible through aerosolized particles and fomites. Amidst a growing volume of patients with coronavirus disease 2019 (COVID-19), the purpose of this study was to evaluate the potential for SARS-CoV-2 transmission through fomites. Samples collected from the exposed skin of clinicians (n = 42) and high-touch surfaces (n = 40) were collected before and after encounters with COVID-19 patients. Samples were analyzed using two assays: the CDC 2019-nCoV Real-Time Reverse Transcription polymerase chain reaction (RT-qPCR) assay, and a SYBR Green assay that targeted a 121 bp region within the S-gene of SARS-CoV-2. None of the samples tested positive with the CDC assay, while two high-touch surface areas tested positive for SARS-CoV-2 using the Spike assay. However, viral culture did not reveal viable SARS-CoV-2 from the positive samples. Overall, the results from this study suggest that SARS-CoV-2 RNA were not widely present either on exposed skin flora or high-touch surface areas in the hospital locations tested. The inability to recover viable virus from samples that tested positive by the molecular assays, however, does not rule out the possibility of SARS-CoV-2 transmission through fomites. |
| Databáze: | OpenAIRE |
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