Continuous positive airway pressure affects mitochondrial function and exhaled PGC1-α levels in obstructive sleep apnea

Autor:	Mei-Wei Lin, Chung-Hsin Chiu, Yi-Kun Sun, Wei-Ji Chen, Ching-Chi Lin, I-Shiang Tzeng
Rok vydání:	2021
Předmět:	Pulmonary and Respiratory Medicine medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Inflammation medicine.disease_cause Gastroenterology Internal medicine medicine Humans Exhaled breath condensate Sleep study Continuous positive airway pressure Receptor Molecular Biology Sleep Apnea Obstructive Continuous Positive Airway Pressure business.industry medicine.disease Mitochondria nervous system diseases respiratory tract diseases Obstructive sleep apnea Oxidative Stress medicine.symptom Airway business Oxidative stress
Zdroj:	Experimental Lung Research. 47:476-486
ISSN:	1521-0499 0190-2148
DOI:	10.1080/01902148.2021.2001607
Popis:	Purpose: Subjects with obstructive sleep apnea (OSA) exhibit systemic and upper airway oxidative stress and inflammation, which cause mitochondrial dysfunction. The intend of this study is to estimate mitochondrial function (mitochondrial DNA/nuclear DNA [Mt/N] ratio) and protein levels of peroxisome proliferator-coactivated receptor gamma co-activator 1-alpha (PGC1-α) in the exhaled breath condensate (EBC) and plasma before and after continuous positive airway pressure (CPAP) treatment. Materials and methods: Twenty healthy individuals (control) and 40 subjects with severe or moderate OSA were recruited to undergo CPAP treatment and evaluation in a sleep study. The Mt/N ratio in the EBC and blood were assayed by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the protein concentration of PGC1-α in the EBC and plasma. All experiments were performed after 3 months of CPAP treatment in subjects with OSA. Results: We observed no noteworthy differences between the control and treatment groups. Moreover, there were no differences in the Mt/N ratio in the blood and plasma levels of PGC1-α in subjects with OSA before and after treatment. However, the Mt/N ratio and protein levels of PGC1-α in the EBC of OSA subjects were higher than those in the control group and returned to normal levels after CPAP treatment. Conclusions: We successfully treated subjects with OSA by CPAP, which restored the Mt/N ratio and levels of PGC1-α in the EBC.
Databáze:	OpenAIRE
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