Proteoglycan from salmon nasal cartridge [corrected] promotes in vitro wound healing of fibroblast monolayers via the CD44 receptor
| Autor: | Yoshie Takeda, Takeshi Kobayashi, Masahiro Sokabe, Gen Ito |
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| Rok vydání: | 2014 |
| Předmět: |
Biophysics
Biochemistry chemistry.chemical_compound Mice Nasal Cartilages Salmon medicine Chondroitin Animals Humans Chondroitin sulfate Aggrecans Fibroblast Molecular Biology Wound Healing biology Cell growth CD44 Chondroitin Sulfates Cell migration Cell Biology Fibroblasts Cell biology medicine.anatomical_structure Hyaluronan Receptors Proteoglycan chemistry biology.protein NIH 3T3 Cells Biological Assay Wound healing |
| Zdroj: | Biochemical and biophysical research communications. 456(3) |
| ISSN: | 1090-2104 |
| Popis: | Proteoglycans (PGs) are involved in various cellular functions including cell growth, adhesion, and differentiation; however, their physiological roles are not fully understood. In this study, we examined the effect of PG purified from salmon nasal cartilage (SNC-PG) on wound closure using tissue-cultured cell monolayers, an in vitro wound-healing assay. The results indicated that SNC-PG significantly promoted wound closure in NIH/3T3 cell monolayers by stimulating both cell proliferation and cell migration. SNC-PG was effective in concentrations from 0.1 to 10μg/ml, but showed much less effect at higher concentrations (100-1000μg/ml). The effect of SNC-PG was abolished by chondroitinase ABC, indicating that chondroitin sulfates (CSs), a major component of glycosaminoglycans (GAGs) in SNC-PG, are crucial for the SNC-PG effect. Furthermore, chondroitin 6-sulfate (C-6-S), a major CS of SNC-PG GAGs, could partially reproduce the SNC-PG effect and partially inhibit the binding of SNC-PG to cells, suggesting that SNC-PG exerts its effect through an interaction between the GAGs in SNC-PG and the cell surface. Neutralization by anti-CD44 antibodies or CD44 knockdown abolished SNC-PG binding to the cells and the SNC-PG effect on wound closure. These results suggest that interactions between CS-rich GAG-chains of SNC-PG and CD44 on the cell surface are responsible for the SNC-PG effect on wound closure. |
| Databáze: | OpenAIRE |
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