| Autor: |
Zehra Omeroglu Ulu, Zeynep Busra Bolat, Fikrettin Sahin |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 73 |
| ISSN: |
1878-3252 |
| Popis: |
Hepatocelular carcinoma is one of the leading cancer types with no effective cure as poor prognosis is still a challenging aspect. Thus, alternative therapeutics are necessary to control hepatocelular carcinoma. Boron derivatives such as boric acid (BA), sodium perborate tetrahydrate (SPT) and sodium pentaborate pentahydrate (NaB) have been discovered to have anti-cancer effect. This study investigated the anti-proliferative effects of SPT against hepatocelular carcinoma (HCC) using in vitro and transcriptome approaches.Cytotoxic level of SPT on cell survival were detected using MTS assay. The apoptotic cell death and cell cycle arrest was determined using Annexin V/PI and cell cycle assay, respectively. Transcriptome analysis was performed using RNA-seq, followed by functional and KEGG pathway enrichment analysis. qPCR was used to validate the different genes.SPT treated HepG2 and Hep3B cells induced cytotoxicity having ICIn vitro experiments and RNA-seq analysis show anti-proliferative and apoptotic effect of SPT in HCC cells. Further studies might help in understanding the molecular mechanisms of SPT. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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