A novel impaction technique to create experimental articular fractures in large animal joints
| Autor: | Thomas E. Baer, Stephen L. Hillis, P. Zhang, Yuki Tochigi, James A. Martin, M.J. Rudert, Thomas D. Brown |
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| Rok vydání: | 2012 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Intra-Articular Fractures Swine medicine.medical_treatment Biomedical Engineering Osteoarthritis Mechanical insult Osteotomy Tarsal Joints Chondrocyte Article 03 medical and health sciences 0302 clinical medicine Chondrocytes Rheumatology In vivo medicine Animals Orthopedics and Sports Medicine 030203 arthritis & rheumatology Intra-articular fracture 030222 orthopedics Cell Death Impaction business.industry Cartilage Tarsal Joint Anatomy Animal modeling medicine.disease Cartilage injury Disease Models Animal medicine.anatomical_structure Fracture (geology) business Chondrocyte death |
| Zdroj: | Osteoarthritis and cartilage. 21(1) |
| ISSN: | 1522-9653 |
| Popis: | summary Objective: A novel impaction fracture insult technique, developed for modeling post-traumatic osteoarthritis in porcine hocks in vivo, was tested to determine the extent to which it could replicate the celllevel cartilage pathology in human clinical intra-articular fractures. Design: Eight fresh porcine hocks (whole-joint specimens with fully viable chondrocytes) were subjected to fracture insult. From the fractured distal tibial surfaces, osteoarticular fragments were immediately sampled and cultured in vitro for 48 h. These samples were analyzed for the distribution and progression of chondrocyte death, using the Live/Dead assay. Five control joints, in which “fractures” were simulated by means of surgical osteotomy, were also similarly analyzed. Results: In the impaction-fractured joints, chondrocyte death was concentrated in regions adjacent to fracture lines (near-fracture regions), as evidenced by fractional cell death significantly higher (P < 0.0001) than in central non-fracture (control) regions. Although nominally similar spatial distribution patterns were identified in the osteotomized joints, fractional cell death in the near-osteotomy regions was nine-fold lower (P < 0.0001) than in the near-fracture regions. Cell death in the nearfracture regions increased monotonically during 48 h after impaction, dominantly within 1 mm from the fracture lines. Conclusion: The impaction-fractured joints exhibited chondrocyte death characteristics reasonably consistent with those in human intra-articular fractures, but were strikingly different from those in “fractures” simulated by surgical osteotomy. These observations support promise of this new impaction fracture technique as a mechanical insult modality to replicate the pathophysiology of human intraarticular fractures in large animal joints in vivo. |
| Databáze: | OpenAIRE |
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